Purification of a protein can be extremely time consuming and involve setting up a complex series of steps for removing several components of a solution / buffer. Both the number and nature of these steps can involve huge losses of protein, making further research more problematic and having major financial implications.

NVoy technology can make purification simpler and more efficient whilst also minimising the losses associated with each step involved. The NVoy Polymer is uncharged and does not interfere with most purification strategies (e.g. ion exchange, affinity and hydrophobic interactions) and can also be completely removed with ease - greater than 95% of NV10 molecules can be removed using just one ion exchange step

NVoy can also minimise losses during a purification strategy by restricting the non-specific binding with resins, columns, plastics, etc and therefore greatly enhance recovery rates.

For example, a simple four-step protocol with a good recovery rate of 70% at each step would still lose 75% of the protein during processing due to the cumulative addition of losses. If use of NV molecules could increase recovery to >90% at each step, you could double the efficiency of the purification (only 35% of the protein would be lost).

Buffer Exchange also plays a vital role in most purification schemes. Replacing common components of buffers (e.g. detergents) which are required for keeping proteins soluble but may be problematic (e.g. inhibit structural analysis) can be vital to reach research milestones. Replacement of detergents with NVoy allows for the analysis of your protein by any chosen methodology and can decrease the number of steps, and therefore proteins losses, during the processing as a whole.

For further information please contact us at: info@expedeon.com