TEV-Express contains an enhanced form of a catalytic fragment of the N1a protein of Tobacco etch virus (TEV), a cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly. TEV-Express does not require any special buffers for its activity and can be used in a buffer most suitable for the target protein. TEV-Express is a 52 kDa protein with both GST and His tags for easy removal using either Amintra NiHIS or Glutathione purification resins.

Gel:  A 49 kDa GST-fusion protein (C) at 1 mg/ml is incubated with TEV-Express or TEV Protease at a ratio of (1) 1:50, (2) 1:100, (3) 1:200 (w/w) in a buffer of 25 mM Tris-HCl, pH8.0, 150 mM NaCl, 14 mM b-mercaptoethanol at 4°C for 16 hours. The cleaved products are 27 kDa and 22 kDa. 'TEV' is a competitors’ TEV Protease product.

3C-Express is a recombinant, restriction grade, HRV3C (Human rhinovirus 3C) protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro, commonly referred to as the PreScission Site, and cleaves between Gln and Gly.  3C-Express has robust activity at 4°C and high specific activity. 3C-Express is a 47 kDa protein with both GST and His tags for easy removal using either Amintra NiHIS or Glutathione purification resins

Gel:  A 68 kDa GST-fusion protein (C) at 1 mg/ml is incubated with 3C-Express (*) at a ratio of (1) 1:50, (2) 1:100, (3) 1:200, (4) 1:400 (w/w) in a buffer of 25 mM Tris-HCl, pH8.0, 150 mM NaCl, 14 mM b-mercaptoethanol at 4°C for 16 hours. The cleaved products are 42 kDa and 26 kDa.

BaseMuncher is a non-specific endonuclease that hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5’-phosphorylated oligonucleotides of 1-4 bases in length. Recombinantly produced in E. coli using the Benzonase gene in a proprietary process , BaseMuncher is a highly purified homodimer of 27 kDa subunits that has exceptionally high specific activity and is completely free of protease activity.  BaseMuncher is ideal for  reducing viscosity during protein purification and sample preparation.