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Order and Expedeon Enzyme in September and you will get a 10% Discount AND a Free 1GB Memory Stick!
Offer Code: ENZUSB.
Offer Ends 30th September 2009. Not available for online.
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TEV-Express
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TEV-Express contains an enhanced form of a catalytic fragment of the N1a protein of Tobacco etch virus (TEV), a cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly. TEV-Express does not require any special buffers for its activity and can be used in a buffer most suitable for the target protein. TEV-Express is a 52 kDa protein with both GST and His tags for easy removal using either Amintra NiHIS or Glutathione purification resins.
Gel: A 49 kDa GST-fusion protein (C) at 1 mg/ml is incubated with TEV-Express or TEV Protease at a ratio of (1) 1:50, (2) 1:100, (3) 1:200 (w/w) in a buffer of 25 mM Tris-HCl, pH8.0, 150 mM NaCl, 14 mM b-mercaptoethanol at 4°C for 16 hours. The cleaved products are 27 kDa and 22 kDa. 'TEV' is a competitors’ TEV Protease product.
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Specific Activity
1 Unit of TEV-Express cleaves >85% of 3 µg of control substrate in 1 hour at 30°C.
Product Information
| Cat# |
Units |
Volume |
| TEV0010 |
1,000 |
50 µl |
| TEV0100 |
10,000 |
500 µl |
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3C-Express
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3C-Express is a recombinant, restriction grade, HRV3C (Human rhinovirus 3C) protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro, commonly referred to as the PreScission Site, and cleaves between Gln and Gly. 3C-Express has robust activity at 4°C and high specific activity. 3C-Express is a 47 kDa protein with both GST and His tags for easy removal using either Amintra NiHIS or Glutathione purification resins
Gel: A 68 kDa GST-fusion protein (C) at 1 mg/ml is incubated with 3C-Express (*) at a ratio of (1) 1:50, (2) 1:100, (3) 1:200, (4) 1:400 (w/w) in a buffer of 25 mM Tris-HCl, pH8.0, 150 mM NaCl, 14 mM b-mercaptoethanol at 4°C for 16 hours. The cleaved products are 42 kDa and 26 kDa.
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Specific Activity
1 Unit of 3C-Express cleaves >95% of 100 mg of target protein at 4°C for 16 hours.
Product Information
| Cat# |
Units |
Volume |
| 3CE0005 |
500 |
250 µl |
| 3CE0050 |
5,000 |
2,500 µl |
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BaseMuncher
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BaseMuncher is a non-specific endonuclease that hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5’-phosphorylated oligonucleotides of 1-4 bases in length. Recombinantly produced in E. coli using the Benzonase gene in a proprietary process , BaseMuncher is a highly purified homodimer of 27 kDa subunits that has exceptionally high specific activity and is completely free of protease activity. BaseMuncher is ideal for reducing viscosity during protein purification and sample preparation.
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| Activity |
Purity |
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| Activity Gel: 50 mg of salmon sperm DNA was incubated with the indicated units of BaseMuncher and another brand of nuclease at 37°C for 30 min in a buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl2. DNA digestion was monitored by agarose gel. |
Purity Gel: SDS-PAGE of 1, 3, 10 and 30 ug BaseMuncher . No impurities visible.
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Specific Activity
1 Unit of BaseMuncher converts 1.0 OD260 of salmon sperm DNA into acid-soluble nucleotides in 30 minutes at 37°C in a reaction buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl2. This corresponds to complete digestion of 50 µg of salmon sperm DNA into oligonucleotides.
Product Information
| Cat# |
Units |
Volume |
| BM0025 |
25,000 |
100 µl |
| BM0100 |
100,000 |
400 µl |
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