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NEW: HIGH capacity Ni-NTA resin with binding capacity in excess of 50mg/ml resin.  Limited 2 for 1 introductory discount on 5ml packs. Offer valid until 31/01/2012. [Click Here]

 
Amintra Purification Resins

Amintra is a range of cost-effective chromatography resins designed for superior purification performance and customer satisfaction compared to the current 'premium' resins.  Amintra Resins are currently available for  purifying His-Tagged & GST-Tagged recombinant proteins and also Antibodies with more in development - watch this space...

Amintra Core Features
  •  High binding capacity
  •  Excellent specificity & resolution
  •  Outstanding durability & stability
  •  High flow rate compatible
  •  Suitable for all types of chromatography
   
HIS-Tagged Protein Purification

Expedeon offers a range of chelating resins for HIS-Tag protein purifaction including high capacity N-NTA resin in addition to regular and rapid flow resins. All resins are precharged with either nickel or cobalt ion and designed for simple and easy purification of recombinant HIS-tagged protein from cell lysate under denaturing or native conditions.

Resin Type    
Description Storage

Shelf Lifed
 Ni-NTA
(ANNxxxx)
 Precharged Nickel NTA on crosslinked 6% agarose with average size of 110 µm . binding capacity: >50mg/ml.  4°C  2 Years
Ni-Rapid Flow
(ANRxxxx)		 Precharged Rapid Flow Nickel affinity resin for simple and fast purification of recombinant HIS-tagged proteins. Beads are supercross linked allowing higher back pressure and flowrates. 4°C  2 Years
Ni-Agarose
(ANIxxxx)		 Precharged Ni affinity resin for simple and rapid purification of recombinant HIS-tagged proteins, consististing of 45-165 µm agarose beads covalently coupled to a chelating group (IDA). Typical binding capacity of 10 mg HIS6x-tagged protein / ml resin. 4°C  2 Years

 

Co-Rapid Flow
 (ACRxxxx)

     		Precharged Rapid Flow cobalt affinity resin for simple and fast purification of recombinant HIS-tagged proteins. Beads are supercross linked allowing higher back pressure and flowrates. 4°C  2 Years

Co-agarose
(ACOxxxx)

 

 Precharged Cobalt affinity resin for simple and rapid purification of recombinant HIS-tagged proteins, consististing of 45-165 µm agarose beads covalently coupled to a chelating group (IDA). Typical binding capacity of 10 mg HIS6x-tagged protein / ml resin.

4°C

 

 

 

                               2 Years
 

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GST-Tagged Protein Purification
Amintra Glutathione Superflow resin has been optimized for rapid, high performance purification of GST-tagged protein. The resin consists 40-165 µm superflow beads covalently coupled to glutathione. Typically binding capacity of 10 mg GST-tagged protein / ml resin.

 
Catalogue Number Description Storage

Shelf Life
AGUT 1 ml Glutathione Resin - supplied as a 50% slurry in Ethanol +4°C  2 Years
AGU0005 5 ml Glutathione Resin - supplied as a 50% slurry in Ethanol +4°C  2 Years
AGU0025 25 ml Glutathione Resin - supplied as a 50% slurry in Ethanol +4°C  2 Years
AGU0100 100 ml Glutathione Resin - supplied as a 50% slurry in Ethanol +4°C  2 Years

 

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Antibody Purification

Amintra Protein A  and Protein G resins are designed on a sepharose fast flow matrix covalently modified with recombinant Protein A  and Protein G expressed in E. coli. The albumin domain naturally present in these proteins has been deleted to create resins with high capacity and affinity for antibodies. Typically binding capacity of 25 mg IgG / ml resin.

Protein A

Catalogue Number Description Storage

Shelf Life
APAT 1 ml Protein A Resin - supplied as a 50% slurry in Ethanol +4°C  2 Years
APA0005 5 ml Protein A Resin - supplied as a 50% slurry in Ethanol +4°C  2 Years
APA0025 25 ml Protein A Resin - supplied as a 50% slurry in Ethanol +4°C  2 Years
APA0100 100 ml Protein A Resin - supplied as a 50% slurry in Ethanol +4°C  2 Years

 

Protein G

Catalogue Number Description Storage

Shelf Life
APGT 1 ml Protein G Resin - supplied as a 50% slurry in Ethanol +4°C  2 Years
APG0005 5 ml Protein G Resin - supplied as a 50% slurry in Ethanol +4°C  2 Years
APG0025 25 ml Protein G Resin - supplied as a 50% slurry in Ethanol +4°C  2 Years
APG0100 100 ml Protein G Resin - supplied as a 50% slurry in Ethanol +4°C  2 Years

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