Product Description:  RunBlue SDS Running Buffer, 20x

Product Code:  NXB50500

• 0.8 M Tricine
• 1.2 M Tris
• 2% SDS
• 50 mM Sodium Bisulfite

Tricine (free base)	71.70 g
Tris (free base)	72.60 g
SDS	10.00 g
Sodium Bisulfite	2.5 g
Ultra pure water to	500 ml (~ 385 g)

The pH should be between 8.2 and 8.3 @ 25°C.

For Non-reduced samples(especially antibodies), omit the Sodium Bisulfite

For best results use low conductance ingredients to formulate the buffers.  Do NOT use acid or base to adjust pH of buffers.

Product Description:  RunBlue LDS Sample Buffer, 4x

Product Code:  NXB31002

• 40% Glycerol
• 4% LDS, 0.8 M Triethanolamine-Cl pH 7.6
• 4% Ficoll-400
• 0.025% Phenol Red
• 0.025% Coomassie Brilliant Blue G250
• 2 mM EDTA-2Na

Glycerol	4.0 g
Triethanolamine	1.2 g
6N HCl	0.93 g
Lithium Dodecyl Sulfate	0.40 g
Ficoll 400	0.40 g
EDTA Di-Sodium	0.007 g
Brilliant Blue G250	0.0025 g
Phenol red	0.0025 g
Ultra pure water to	10 ml (~4.5 g)

The pH should be between 7.7 and 7.8 @ 25°C.

For best results use low conductance ingredients to formulate the buffers. 

Do NOT use acid or base to adjust pH of buffers.

Product Description:  RunBlue Native Running Buffer, 20x

Product Code:  NXB61500

• 0.8 M Tricine
• 1.2 M Tris

Tricine (free base)	71.70 g
Tris (free base)	72.60 g
Ultra pure water to	500 ml (~ 385 g)

The pH should be around 8.3 @ 25°C.

For best results use low conductance ingredients to formulate the buffers. 

Do NOT use acid or base to adjust pH of buffers.

Product Description:  RunBlue Native Sample Buffer, 4x

Product Code:  NXB33010

• 40% Glycerol
• 4% Ficoll-400,
• 0.8 M Triethanolamine-Cl pH 7.7
• 0.025% Phenol Red
• 0.025% Coomassie Brilliant Blue G250
• 2 mM EDTA-2Na

Glycerol	4.0 g
Triethanolamine	1.2 g
6N HCl	0.93 g
Ficoll 400	0.40 g
EDTA Di-Sodium	0.007 g
Brilliant Blue G250	0.0025 g
Phenol red	0.0025 g
Ultra pure water to	10 ml (~4.5 g)

The pH should be between 7.7 and 7.8 @ 25°C.

For best results use low conductance ingredients to formulate the buffers. 

Do NOT use acid or base to adjust pH of buffers.

Product Description:  RunBlue TGS Blot Buffer, 10x / 20xStock Solution

Product Code:  NXB82500

• 1.92 M Glycine
• 0.25 M Tris
• 1% SDS

The pH should be between 8.5 and 8.6 @ 25°C.

For best results use low conductance ingredients to formulate the buffers. 

Do NOT use acid or base to adjust pH of buffers.

Instructions:  Prior to use, dilute this blot buffer stock solution:

• 10x for use in RunBlue Dual Run & Blot System of semi-dry blotters.
• 20x for use in Tank Blotters and for the Invitrogen XCell II Blot Module