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RunBlue Bis-Tris FAQs

February 27th, 2018

Do you know why the gel took 3 hours to run half way? Check whether the appropriate running buffer to separate the proteins is used. Expedeon Bis-tris gels, along with the NuPAGE gels, need MES or MOPS running buffer to separate the proteins. They allow proteins to run slower. Are Expedeon Bis Tris gels larger…

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InstantBlue Protein Stain FAQs

February 27th, 2018

What is the sensitivity of InstantBlue? It can detect as low as 5 ng of protein (BSA). Does InstantBlue has an upper limit of detection? The stain does have an upper limit dependent on the volume of the InstantBlue Stain used. The more InstantBlue, the more Coomassie dye that is free to bind to protein….

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