Lyophilized SunScript® RT

March 20th, 2019

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CACLP

20 – 24 March 2019

FFPE-FASP™ Protein Digestion Kit FAQ

March 11th, 2019

What Does the FFPE-FASP Protein Digestion Kit Do? The FFPE-FASP Protein Digestion Kit: • Reverses protein crosslinking that originates from formalinfixation • Extracts the proteins from the tissue • Reduces the disulfide bonds • Depletes contaminants from any protein sample solution • Alkylates thiols • Digests proteins in a spin-filter “proteomic reactor” • Releases peptide…

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Protein Electrophoresis (Western Blotting) Technical Handbook

March 8th, 2019

Electrophoresis is a simple, rapid, and highly sensitive analytical technique to study the properties of proteins and nucleic acids, and has become a principle tool in analytical chemistry, biochemistry, and molecular biology. Electrophoresis is a particularly useful way to separate proteins prior to downstream detection or analysis (e.g. Western blot) and is a critical step…

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Precast vs. Handcast Gels for SDS PAGE

March 5th, 2019

Precast Gels Offer Better Data Quality Most researchers use precast gels; this way they can readily generate consistent and reproducible data, as well as benefit from significant time savings. Precast gels are now available in a wide variety of percentages and formulations, including gradient gels for the resolution of a broader range of proteins. Precast…

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Tris-Glycine vs. Bis-Tris Gel Chemistry

March 5th, 2019

Tris-Glycine Chemistry The Tris-Glycine gel formulation, based on the Laemmli system, is the simplest and most widely used system for separating a broad range of proteins by SDS PAGE and native PAGE (i.e., without SDS or alternative denaturant). The system utilizes two Tris-Glycine gels; the first is a stacking gel that helps focus the proteins…

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