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Antibody Barcoding Kits

Just 30 seconds hands-on time, introducing a faster and more efficient way of barcoding antibodies with oligonucleotides.

Start exploring the full potential of single-cell multi-omics!

What are barcoded antibodies?

Image containing a schematic depiction of an antibody conjugated to an oligonucleotide (barcoded antibody)
PCR handle – a primer for sequencing
Feature barcode – unique for each antibody
Capture sequence – complementary to the oligo on the Gel Bead

Antibody barcoding – an important step in evaluating gene expression and protein levels simultaneously.

Recently developed techniques such as CITE-seq, REAP-seq and Cell hashing, are becoming increasingly popular for exploring single-cell multi-omic approaches. By combining barcoded antibodies with scRNA-seq platforms (i.e.10x Genomics Chromium, BD Rhapsody™ Single-Cell Analysis System, Fluidigm C1, Drop-seq, Clontech iCell8 and ddSEQ™ ) we are now able to correlate transcriptomics data with the actual protein expression in a single cell.

However, one of the biggest challenges with these techniques is how to efficiently conjugate oligonucleotides to antibodies

With Lightning-Link® antibody barcoding kits you can conjugate your preferred antibody to the pre-activated oligo mix with just 30 seconds hands-on time. This will enable you to explore more parameters at a single-cell level while not being limited by commercially available antibody-oligo conjugates.

antibody_barcoding_worklow_image

Lightning-Link® antibody barcoding kit workflow

If you’d like to receive updates about Lightning-Link® antibody barcoding kit, please register by filling out the form on the right-hand side of this page.

Can’t wait for it?

You might also be interested in our Thunder-Link® PLUS Oligo Conjugation System. It enables simple and rapid conjugation of antibodies to oligonucleotides at a desired ratio, with high recovery of materials and a superior clean up procedure.

References:

If you want to see how Thunder-Link® PLUS Oligo Conjugation System helped other researchers to get more information out of a single cell, check out these peer-reviewed publications:

Marlon Stoeckius, Christoph Hafemeister, William Stephenson, Brian Houck-Loomis, Pratip K Chattopadhyay, Harold Swerdlow, Rahul Satija & Peter Smibert, Simultaneous epitope and transcriptome measurement in single cells, Nature Methods volume 14, pages 865–868 (2017)

Peterson, V. M., Zhang, K. X., Kumar, N., Wong, J., Li, L., Wilson, D. C., Klappenbach, J. A. (2017). Multiplexed quantification of proteins and transcripts in single cells. Nature Biotechnology, 35(10), 936–939.doi:10.1038/nbt.3973 

Govek K. W., Troisi E. C., Woodhouse S., Camara P. G. (2019). Single-Cell Transcriptomic Analysis of mIHC Images via Antigen Mapping. bioRxiv 672501; doi: https://doi.org/10.1101/672501