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Product Applications & Technical Notes

Precast vs. Handcast Gels for SDS PAGE

March 5th, 2019

Precast Gels Offer Better Data Quality Most researchers use precast gels; this way they can readily generate consistent and reproducible data, as well as benefit from significant time savings. Precast gels are now available in a wide variety of percentages and formulations, including gradient gels for the resolution of a broader range of proteins. Precast…

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Tris-Glycine vs. Bis-Tris Gel Chemistry

March 5th, 2019

Tris-Glycine Chemistry The Tris-Glycine gel formulation, based on the Laemmli system, is the simplest and most widely used system for separating a broad range of proteins by SDS PAGE and native PAGE (i.e., without SDS or alternative denaturant). The system utilizes two Tris-Glycine gels; the first is a stacking gel that helps focus the proteins…

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His-Tag Protein Production and Purification

December 5th, 2018

Contents Introduction Purification of His-Tag Proteins Detection of His-Tag Proteins Easy Validation of His-Tag Protein Expression Related Products References Products His-Tag Check&Go! ULFA 40nm InnovaCoat® GOLD Amintra™ Ni-NTA affinity resin Amintra™ Cobalt NTA Resin Amintra™ Nickel Magnetic AminTRAP HIS Prepacked Columns Related Reading Affinity Chromatography SDS PAGE Western blot – Application Note Immunoprecipitation Immunochromatography Introduction The…

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GELFrEE 8100 Fractionation System

October 5th, 2018

GELFrEE Fractionation for Sample Preparation Successful sample preparation is a key step during any analytical procedure and begins with a defined experimental design. Important steps in sample preparation include proteolytic digestion of proteins into peptide fragments, and peptide fractionation. This is especially important prior to applications such as mass spectrometry (MS). For “bottom up” proteomics,…

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Antibody–Oligonucleotide (Ab–Oligo) Conjugate Preparation and Applications

October 3rd, 2018

Antibody–Oligonucleotide Conjugates Introduction Antibody–oligonucleotide (Ab–Oligo) conjugates have been used in numerous applications from diagnostics to therapeutics and were developed through an unmet need for precise and efficient detection of low-abundance proteins. Ab–Oligo conjugates have since played a significant role in enhancing an extensive range of biological techniques that include immunological and proteomic research, biomarker discovery,…

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Labeling Non-Antibody Proteins and Small Molecules – An Advanced Guide

June 18th, 2018

Lightning-Link® Conjugation with Non-Antibody Proteins and Small Molecules The Lightning-Link conjugation system is the world’s easiest way of making conjugates with any one of around 50 labels. Lightning-Link was originally optimized for antibodies, but it has a much broader range of application. The following guide may help if you wish to label molecules other than…

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