Application notes

Labeling non-antibody proteins and small molecules – An Advanced Guide

June 18th, 2018

Lightning-Link® conjugation with non-antibody proteins and small molecules The Lightning-Link conjugation system is the world’s easiest way of making conjugates with any one of around 50 labels. Lightning-Link was originally optimized for antibodies, but it has a much broader range of application. The following guide may help if you wish to label molecules other than…

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Fluorescent detection in lateral flow

October 12th, 2017

Lateral flow assays, also known as immunochromatographic assays or strip tests, are immunoassays which are used to detect the presence, or absence, of a target analyte in a sample. Since they are suitable for point-of-care testing, provide a result extremely quickly, and offer simple, user-friendly operation they are rapidly growing in popularity. To find out…

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Immunohistochemistry (IHC) – direct labeling

August 28th, 2017

Lightning-Link® antibody labeling kits can be used to directly conjugate antibodies for IHC to HRP or other labels. The IHC data below shows that it is possible to obtain very good staining whilst avoiding the use of secondary antibodies, therefore saving significantly in time and reagent costs. Anti-human CD20 (clone L26) was conjugated to HRP…

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