Product Applications & Technical Notes

Precast vs. Handcast Gels for SDS PAGE

March 5th, 2019

Precast Gels Offer Better Data Quality Most researchers use precast gels; this way they can readily generate consistent and reproducible data, as well as benefit from significant time savings. Precast gels are now available in a wide variety of percentages and formulations, including gradient gels for the resolution of a broader range of proteins. Precast…

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Tris-Glycine vs. Bis-Tris Gel Chemistry

March 5th, 2019

Tris-Glycine Chemistry The Tris-Glycine gel formulation, based on the Laemmli system, is the simplest and most widely used system for separating a broad range of proteins by SDS PAGE and native PAGE (i.e., without SDS or alternative denaturant). The system utilizes two Tris-Glycine gels; the first is a stacking gel that helps focus the proteins…

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His-Tag Protein Production and Purification

December 5th, 2018

Contents Products Related Reading Introduction His-Tag Check&Go! Affinity Chromatography Purification of His-Tag Proteins ULFA SDS PAGE Detection of His-Tag Proteins 40nm InnovaCoat® GOLD Western blot – Application Note Easy Validation of His-Tag Protein Expression Amintra™ Ni-NTA affinity resin Immunoprecipitation Related Products Amintra™ Cobalt NTA Resin Immunochromatography References Amintra™ Nickel Magnetic AminTRAP HIS Prepacked Columns Introduction The…

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GELFrEE 8100 Fractionation System

October 5th, 2018

GELFrEE Fractionation for Sample Preparation Successful sample preparation is a key step during any analytical procedure and begins with a defined experimental design. Important steps in sample preparation include proteolytic digestion of proteins into peptide fragments, and peptide fractionation. This is especially important prior to applications such as mass spectrometry (MS). For “bottom up” proteomics,…

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Antibody–Oligonucleotide (Ab–Oligo) Conjugate Preparation and Applications

October 3rd, 2018

Download PDF Antibody–Oligonucleotide Conjugates Introduction Antibody–oligonucleotide (Ab–Oligo) conjugates have been used in numerous applications from diagnostics to therapeutics and were developed through an unmet need for precise and efficient detection of low-abundance proteins. Ab–Oligo conjugates have since played a significant role in enhancing an extensive range of biological techniques that include immunological and proteomic research,…

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Labeling Non-Antibody Proteins and Small Molecules – An Advanced Guide

June 18th, 2018

Lightning-Link® Conjugation with Non-Antibody Proteins and Small Molecules The Lightning-Link conjugation system is the world’s easiest way of making conjugates with any one of around 50 labels. Lightning-Link was originally optimized for antibodies, but it has a much broader range of application. The following guide may help if you wish to label molecules other than…

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Capal

TruePrime® Single Cell WGA Kit

TruePrime® Single Cell WGA Kit

I've used this kit for amplification of DNA from single flow-sorted plant chromosomes. Performance was exactly as desribed, on average I've obtained more than microgram…

Andrea Rawlings

Lightning-Link® Rapid Biotin - Type A

Lightning-Link® Rapid Biotin - Type A

This product worked very effectively and produced a good level of biotinylation. The instructions were easy to follow, and the reaction was fast and reliable.

Boyan Grigorov

InstantBlue™ Protein Stain

InstantBlue™ Protein Stain

We have tried InstantBlue in order to have a rapid result and, yes, it works for 15 minutes as mentioned in the protocol. Before, students…

Alistair

Lightning-Link® R-Phycoerythrin (R-PE)

Lightning-Link® R-Phycoerythrin (R-PE)

The procedure was very simple and gave excellent results

Amy

Lightning-Link® Rapid FluoProbes647H

Lightning-Link® Rapid FluoProbes647H

This kit was very easy to use, and the process of labeling my antibodies is quick. Because I used commercially purchased antibodies, I first had…

Andrea Rawlings

RunBlue™ Bis-Tris Protein Gels

RunBlue™ Bis-Tris Protein Gels

The RunBlue 4-12% gels give very good resolution of our protein bands. They are so convenient to use compared to casting our own gels, and…

Andrea Rawlings

AminTRAP HIS Prepacked Columns

AminTRAP HIS Prepacked Columns

We use the AminTrap nickel columns for purifying 6xHis tagged proteins using an automated purification system. The columns are completely compatible to our system and…

Adel Serwy

excellent,good knowledge.

Andreas

InstantBlue™ Protein Stain

InstantBlue™ Protein Stain

InstantBlue is more sensitive and faster than Coomassie blue stain. You could see clear bands after 10 minutes of incubation. Overnight incubation with InstantBlue is…

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