Product Applications & Technical Notes

His Tag Protein Production and Purification

December 5th, 2018

Contents Introduction Purification of His Tag Proteins Detection of His Tag Proteins Easy Validation of His Tag Protein Expression Related Products References Products His Tag Check&Go! ULFA 40nm InnovaCoat® GOLD Amintra™ Ni-NTA affinity resin Amintra™ Cobalt NTA Resin Amintra™ Nickel Magnetic AminTRAP HIS Prepacked Columns Related Reading Affinity Chromatography SDS PAGE Western blot – Application Note…


GELFrEE 8100 Fractionation System

October 5th, 2018

GELFrEE Fractionation for Sample Preparation Successful sample preparation is a key step during any analytical procedure and begins with a defined experimental design. Important steps in sample preparation include proteolytic digestion of proteins into peptide fragments, and peptide fractionation. This is especially important prior to applications such as mass spectrometry (MS). For “bottom up” proteomics,…


Antibody-Oligonucleotide (Ab-Oligo) Conjugate Preparation and Applications

October 3rd, 2018

Antibody-Oligonucleotide Conjugates Introduction Antibody-oligonucleotide (Ab-Oligo) conjugates have been used in numerous applications from diagnostics to therapeutics and were developed through an unmet need for precise and efficient detection of low-abundance proteins. Ab-Oligo conjugates have since played a significant role in enhancing an extensive range of biological techniques that include immunological and proteomic research, biomarker discovery,…


Pre-Cast vs Hand-Cast Gels for SDS-PAGE

July 31st, 2018

Hand-cast gels can delay workflows Hand-cast gels are time-consuming to prepare and, when poured badly, result in irregular bands or streaking of proteins. This makes quantitation difficult and can delay workflows while experiments are repeated. Further disadvantages of hand-casting include the high potential for introducing user-bias and the susceptibility of hand-casting apparatus to leakage. There…


Labeling Non-Antibody Proteins and Small Molecules – An Advanced Guide

June 18th, 2018

Lightning-Link® Conjugation with Non-Antibody Proteins and Small Molecules The Lightning-Link conjugation system is the world’s easiest way of making conjugates with any one of around 50 labels. Lightning-Link was originally optimized for antibodies, but it has a much broader range of application. The following guide may help if you wish to label molecules other than…