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Product Applications & Technical Notes

Fluorescent Detection in Lateral Flow Assays

October 12th, 2017

Lateral flow assays, also known as immunochromatographic assays or strip tests, are immunoassays which are used to detect the presence, or absence, of a target analyte in a sample. Since they are suitable for point-of-care testing, provide a result extremely quickly, and offer simple, user-friendly operation they are rapidly growing in popularity. To find out…

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Immunohistochemistry (IHC) – Direct labeling

August 28th, 2017

Lightning-Link® antibody labeling kits can be used to directly conjugate antibodies for IHC to HRP or other labels. The IHC data below shows that it is possible to obtain very good staining whilst avoiding the use of secondary antibodies, therefore saving significantly in time and reagent costs. Anti-human CD20 (clone L26) was conjugated to HRP…

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Dye-based detection of inorganic phosphate

August 25th, 2017

Dyes such as Malachite Green are regularly used to detect inorganic phosphate (Pi), an important inorganic ion in a wide variety of biological functions. In the presence of Pi these reagents change color, and a simple absorbance reading can be employed to measure the amount of Pi which has been released from a substrate.  …

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Antibody Labeling Chemistries

August 25th, 2017

Expedeon – easy to use antibody, protein and peptide labeling technologies Anyone new to this field will find a bewildering number of antibody labeling protocols in the literature. It is difficult for the newcomer to know what the critical parameters are and what procedure should be applied in any given situation. With traditional labeling methods,…

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HRP antibody labeling protocol

August 25th, 2017

Directly label your primary antibody with HRP Horseradish peroxidase (HRP) is a 44kDa glycoprotein with 6 lysine residues which can be conjugated to antibodies and proteins for use in a variety of applications. The enzyme label can be visualized through chromogenic reactions; for example diaminobenzidine (DAB) in the presence of hydrogen peroxide (H202) is converted…

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Antibody purification kit selection

August 25th, 2017

Expedeon specializes in easy to use bioconjugation kits which enable the direct labeling of antibodies or proteins with enzymes, fluorescent labels, biotin, streptavidin, gold nanoparticles, latex beads or oligonucleotides. Unfortunately, many antibodies are provided in buffers which contain additives that are incompatible with labeling technologies, making purification a key consideration prior to carrying out any…

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PiColorLock™ applications

August 24th, 2017

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Fluorescent detection in lateral flow

August 9th, 2017

Lateral flow assays, also known as immunochromatographic assays or strip tests, are immunoassays which are used to detect the presence, or absence, of a target analyte in a sample. Since they are suitable for point-of-care testing, provide a result extremely quickly, and offer simple, user-friendly operation they are rapidly growing in popularity. To find out…

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Multi-color flow cytometry

August 8th, 2017

Directly label primary antibodies for multi-color flow cytometry Flow cytometry is an extremely powerful technique that is used to analyze the characteristics of cells as they flow singly past one or more beams of focused light, provided by lasers. When the cells pass through the laser beam they scatter the light, which is detected as…

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