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Product Applications & Technical Notes

Tips For Performing Immuno-PCR

August 8th, 2017

What is Immuno-PCR? Immuno-PCR is an extremely powerful technique that combines the specificity of an enzyme linked immunosorbent assay (ELISA) with the signal amplification of the polymerase chain reaction (PCR). Antibodies specific to the protein target are immobilized onto a microplate, the analyte is captured, and then a second specific antibody which has been coupled…

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The efficiency of conjugation reactions

August 8th, 2017

There are several reasons why you would want to measure conjugation efficiency, with both qualitative and more labour-intensive quantitative methods available. In many cases a qualitative answer may be sufficient, for example to provide reassurance before embarking on a lengthy set of experiments with a new conjugate; but where absolute consistency from batch to batch…

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Improving the efficiency of antibody conjugation to latex beads

August 7th, 2017

Latex beads can be used in a wide range of applications, but are especially popular for lateral flow assays and latex agglutination experiments. Lateral flow assays Lateral flow assays (LFA) are used to quickly and easily establish whether a target analyte is present in a test sample. In a typical LFA, the sample is added…

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The use of carrier proteins to boost the immune response

August 7th, 2017

A hapten is a small molecule, typically with a molecular weight of less than 1-5kDa, which stimulates an immune response resulting in the production of antibodies only when it is attached to a larger carrier molecule. It is unable to generate an immune response on its own, since its size and lack of complexity preclude…

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Using Lightning-Link® conjugates for Immunocytochemistry

September 7th, 2016

Using Lightning-Link® conjugates for Immunocytochemistry – references Directly labeling primary antibodies using Lightning-Link® kits produces conjugates that can be used in a variety of scientific applications. Take a look at some of the published research in the table below that has used Lightning-Link® technology to produce conjugates for use within Immunocytochemistry… Title Pubmed ID Label…

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Improving Immuno-PCR

Improving Immuno-PCR by optimizing Antibody-Oligo Conjugation

March 12th, 2016

Improving Immuno-PCR by optimizing Antibody-Oligo Conjugation – Article from IDT Thursday, 12 March 2015 10:44 Integrated DNA Technologies (IDT), specialists in oligonucleotide synthesis, have put together this great article explaining Immuno-PCR, how we have simplified antibody-oligonucleotide conjugation with our Thunder-Link conjugation technology and the importance of high quality oligo synthesis for highest quality oligos. View…

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