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Protein Electrophoresis

Expedeon have pioneered a number of developments in the field of pre-cast gels and protein electrophoresis, and continue to innovate with new developments in the field. InstantBlue protein stain is a well-established fast acting stain for polyacrylamide gels giving results in minutes.  RunBlue pre-cast gels offer high performance and resolution and are available with a range of chemistries, well numbers, and sizes. Buffers, Molecular Weight markers and Gel Running Tanks are also available.

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Western Blotting

Browse a range of Western blotting reagents, equipment and detection agents. Expedeon has a variety of equipment and consumables for Western Blotting, such as LumiBlue ECL solutions. These solutions are available for a range of protein and antibody concentrations, from routine housekeeping blotting to Western blots requiring the upmost sensitivity. Other specific reagents include pIMAGO detection technology for unbiased detection of phosphorylation events and the Anti-Blue Antibody, which is targeted to blue stained proteins present in pre-stained protein marker.

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Proteomics

Large scale studies of proteins requires a variety of specialised reagents and equipment. Expedeon stocks a wide range of equipment and consumables to streamline proteomics related processes such as sample fractionation, digestion for mass spectrometry and protein extraction and solubilisation. Products range from the Gelfree 8100, a device which can fractionate samples by molecular weight into liquid fractions, to a variety of digestion kits and sample preparation technologies. 

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Protein purification

Recombinant tagging of proteins of interest allows complex protein mixtures or lysates to easily be separated to very high levels of purity, often with only a single purification step. Expedeon have a variety of magnetic and non-magnetic resins for purifying widely used recombinant tags such as histidine, streptavidin, and glutathione tags. Protein A, G, and L resins for purification of antibodies are also available, as well as empty columns and a range of other resins for a number of purification needs.

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Structural Biology

Understanding the molecular structure of biological macromolecules is a key part of truly understanding their function. Expedeon offers a range of novel technologies to help study protein structure and overcome common hurdles involved in structural biology experiments. NVoy technology can be used to stabilise hydrophobic proteins and protect from aggregation in solution, allowing in depth biochemical and biophysical studies that would otherwise be impossible. It can be used for stabilisation and solubilisation of protein without detergents, fusion proteins or chaperones. The VersaWave spectrophotometer can be used for spectrographic investigations of protein over a wide wavelength range.

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Sample preparation

Kits and reagents for protein fractionation, extraction, solubilisation and specific enrichment of samples for a range of downstream processing applications. These products reduce the time spent on repetitive sample preparation protocols while improving accuracy and consistency of results. 

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Protein quantification

Expedeon offers a range of Bradford solutions with enhancements to overcome the typical limitations of the Bradford assay for total protein concentration. As an example, Bradford Ultra allows protein quantitation in an environment containing up to 1% detergent, which is not achievable using conventional Bradford Assays. BCA assays are also available which allow a modification for protocols not requiring reducing agents.

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Cell Signalling

Phosphorylation is a key mechanism for cell signalling and activation of enzymes, and plays a significant role in a huge range of molecular processes.  Expedeon has kits for phosphoprotein detection using both Western blot and microplate techniques. Phosphopeptide isolation can be achieved using PolyMAC capture kits which offer high recovery and unbiased binding of phosphorylated pepetides.

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