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His-Tag Purification

Poly-Histidine tags have been widely employed as a powerful separation approach in the purification of a broad range of proteins and peptides. Amintra His-tag resins are employed for the purification of histidine-tagged recombinant proteins expressed in bacteria, yeast and mammalian cells. A resin for use in columns and a magnetic resin for batch purification are both available.

Catalog #
Name
Unit Size
MSDS
Product Manual
List Price
Qty
Custom Volume
$0.00
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25 ml medium
$225.00
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10 ml medium
$95.00
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Glutathione Tag Purification

Poly-Histidine tags have been widely employed as a powerful separation approach in the purification of a broad range of proteins and peptides. Amintra His-tag resins are employed for the purification of histidine-tagged recombinant proteins expressed in bacteria, yeast and mammalian cells. A resin for use in columns and a magnetic resin for batch purification are both available.

Catalog #
Name
Unit Size
MSDS
Product Manual
List Price
Qty
10 ml medium
$260.00
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25 ml medium
$500.00
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100 ml medium
$1,560.00
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Biotin Tag resin

Amintra Streptavidin Resin can be used to bind biotinylated biomolecules in a variety of batch- or column-type affinity procedures with low non-specific binding.

Catalog #
Name
Unit Size
MSDS
Product Manual
List Price
Qty
2ml medium
$351.00
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5ml medium
$608.00
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10ml medium
$1,051.00
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Tag cleavage enzymes

Expedeons 3C-Express is a recombinant, restriction grade, HRV3C (Human rhinovirus 3C) protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro, commonly referred to as the PreScission Site, and cleaves between Gln and Gly. TEV-Express contains an enhanced form of a catalytic fragment of the N1a protein of Tobacco etch virus (TEV), a cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly. Each enzyme is optimised for high activity over a range of conditions.

Catalog #
Name
Unit Size
MSDS
Product Manual
List Price
Qty
500 Units in 250 ul
$268.00
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5000 Units in 2500 ul
$2,112.00
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1000 Units in 50 ul
$182.00
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Antibody Purification

A number of different recombinant proteins have been shown to bind antibodies with high affinity. These proteins can be used to purify antibodies in column and batch modes. Expedeon offer a range of different magnetic and non-magnetic resins for antibody purification with high binding capacity.

Catalog #
Name
Unit Size
MSDS
Product Manual
List Price
Qty
2ml of 20% slurry (0.2ml medium)
$185.00
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10ml medium
$1,659.00
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5ml medium
$1,030.00
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Affinity Purification

Amintra offers NHS-activated resins and Heparin resins. NHS-activated is a highly cross-linked N-hydroxysuccinimide activated matrix. Ligands containing primary amino groups are coupled directly to this active ester to form a chemically stable amide bond to allow purification of target proteins based on specific interactions. Amintra Heparin Resin is an affinity chromatography medium that is used for the purification of biomolecules that show affinity to heparin, such as antithrombin III, coagulation factors and other plasma proteins.

Catalog #
Name
Unit Size
MSDS
Product Manual
List Price
Qty
10 ml of 50% slurry (5ml medium)
$144.00
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50 ml of 50% slurry (25ml medium)
$309.00
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50 ml of 50% slurry (25ml medium)
$237.00
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Columns

Expedeon provides columns for purification including empty spin or gravity columns for use with any resin and desalting spin columns for sample preparation. 

Catalog #
Name
Unit Size
MSDS
Product Manual
List Price
Qty
pack of 50
$361.00
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pack of 100
$629.00
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(0.5-2ml) - pack of 50 medium
$248.00
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Nucleic Acid Digestion

BaseMuncher is a non-specific endonuclease that hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5-phosphorylated oligonucleotides of 1-4 bases in length. Recombinantly produced in E. coli using the Benzonase gene in a proprietary process, BaseMuncher is a highly purified homodimer of 27 kDa subunits that has exceptionally high specific activity and is completely free of protease activity.  BaseMuncher is ideal for reducing viscosity during protein purification and sample preparation. 

Catalog #
Name
Unit Size
MSDS
Product Manual
List Price
Qty
25,000U (in 100µl)
$279.00
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100,000U (in 2 x 200µl)
$958.00
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Gelfree 8100

The Gelfree 8100 is a piece of bench top equipment for fractionation of complex protein samples by molecular weight. Up to 8 samples can be run in parallel and fractions are recovered in liquid phase for flexibility during downstream processing. The system provides robust fractionation over the mass range 3.5 kDa - 500 kDa with high loading capacity, excellent reproducibility, and over 80% protein recovery after fractionation.

Catalog #
Name
Unit Size
MSDS
Product Manual
List Price
Qty
1 Unit + Cartridges
$20,000.00
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Nvoy Protein Refolding

NVoy refolding kits that provide a simple, generic method for refolding target proteins without the need to screen multiple refolding conditions. NVoy Protein Refolding Kits often produce higher yields of fully refolded protein as NVoy protects the vulnerable intermediates in the refolding process. Furthermore, with NVoy being removable in a slow, time dependant and user controllable manner, the protein is provided enough time and protection to refold correctly.

Catalog #
Name
Unit Size
MSDS
Product Manual
List Price
Qty
6 x 10mg NVOY
$371.00
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6 x 40mg NVOY
$1,051.00
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