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Western Blot Troubleshooting and Tips

December 4th, 2018

Contents Introduction Related Products from Expedeon Weak or No Signal High Background and Nonspecific Bands Other Western Blot Problems References Products Western blotting Systems Buffers and Staining Solutions Blotting Membranes LumiBlue ECL Solutions Protease Inhibitors Lightning-Link® AP Kits   Related Reading Immunoprecipitation Fractionation An Introduction to Gel Electrophoresis Western Blot Application Note     Introduction…

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Lightning-Link® Fluorescein and Lightning-Link® PE/Cy5 in a study of virulence factors

November 13th, 2018

Our Lightning-Link® Fluorescein and Lightning-Link® PE/Cy5 conjugation kits have recently been published! Contents Introduction Results Summary and Conclusion Reference Products Lightning-Link® Fluorescein Lightning-Link® PE/Cy5.5   Related Reading ELISA Flow Cytometry   Introduction Neisseria meningitidis is a commensal bacterium that is usually a harmless resident of the human nasopharynx. However, it has the capability to cause…

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Thunder-Link® PLUS Conjugation System in a Multiplexed Study

November 1st, 2018

Our Thunder-Link® PLUS Conjugation System has recently been published! Contents Introduction Results Summary and Conclusion Use of Thunder-Link® Reference Products Thunder-Link® PLUS Conjugation System . Related Reading Flow Cytometry Immuno-PCR Proximity Ligation Assay Introduction RNA expression and protein sequencing assay (REAP-seq) is a tool that increases the throughput and simultaneous measurement of gene and protein…

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Lightning-Link® R-Phycoerythrin in a Preclinical Study for Acute Myeloid Leukemia

October 18th, 2018

Our Lightning-Link® R-Phycoerythrin (RPE) Conjugation Kit has recently been published! Contents Introduction Results Summary and Conclusion Use of Lightning-Link® RPE Conjugation Kit to Establish Expression of Cell-Surface GRP78 References Products Lightning-Link R-Phycoerythrin (R-PE) Kit Related Reading Flow Cytometry (FACS) Introduction Glucose-regulated protein-78 (GRP78) is a stress-response chaperone that has been found to be associated with…

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Lightning-Link® Horseradish Peroxidase in a rat model of renal interstitial fibrosis

October 2nd, 2018

Our Lightning-Link® Horseradish Peroxidase (HRP) conjugation kit has recently been published! Introduction Renal interstitial fibrosis (IF) is an important pathological feature of disease progression in chronic kidney disease (CKD). However, early detection and quantitative analysis of markers for IF due to CKD progression remains a challenge. Early detection of renal tissue remodelling prior to commencement…

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Liquid Biopsy, Key for Precision Medicine

July 24th, 2018

Sequencing Circulating Tumor DNA Can Provide a More Complete Molecular Picture The last 10 years have seen enormous progress in both the understanding of cancer as a disease, as well as the development of targeted therapies, with an unprecedented number of new drugs approved for cancer treatment by the FDA and EMA. The availability of…

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What people say about our tech support

February 15th, 2018

Here are few comments that our customers made on our tech support team:   “Expedeon technical support team has supported me in my experiments by helping and guiding me. The response time has been very very fast and the information provided very accurate!”   “Expedeon technical support staff was extremely helpful. Even though my inquiry was…

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Strip test to diagnose Sickle Cell Anemia

February 5th, 2018

Introduction Sickle cell anemia (SCA)is caused by a mutation of the beta globin gene. It affects about 300,000 newborns each year, and includes symptoms such as severe anemia, pain and decreased immune function. Individuals who are homozygous for the sickle cell gene mutation produce only HbS (genotype SS) are affected by the disease whereas heterozygotes,…

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Streamline & expand your antibody manufacturing services

February 5th, 2018

Our Virtual Library concept can provide antibody manufacturers the unique opportunity to rapidly increase their antibody conjugate catalogue whilst avoiding the difficulties presented by the need to hold large inventories of items that do not have any sales history. How can our Virtual Library help you? A complete virtual model Avoid expensive inventory costs with…

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SunScript™

February 2nd, 2018

SunScript™ Reverse Transcriptase Basis of the Reverse Transcription Reaction SunScript™ Reverse Transcriptase allows reaction temperatures up to 85°C 100 ng of total RNA from HeLa cells were reverse transcribed at the indicated temperatures for 60 min with SunScript™ Reverse Transcriptases and Oligo(dT)18 primer, followed by inactivation at 95°C for 10 min. Presence of GAPDH cDNA…

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TruePrime™

February 2nd, 2018

How does TruePrime™ technology work?   TruePrime™: absence of primer artefacts 1 pg of human genomic DNA (~ 1/6 of the content of one human/mammalian cell) has been amplified using either TruePrime™ (TthPrimPol-based MDA) or random primed MDA reactions. Random primed reactions contain 20% of sequences that cannot be mapped to any organism in sequence…

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Bis-Tris Gels Technical Information

February 1st, 2018

RunBlue® Bis-Tris Protein Gels have been developed by Expedeon™ to provide an alternative to NuPAGE® Bis-Tris gels. These protein gels run with the same voltages, in the same tanks, using the same MES/MOPS buffers as NuPAGE gels. RunBlue Bis-Tris gels achieve highly comparable results with exceptional reproducibility and resolution. Polyacrylamide gels are based on the…

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