Blog

What people say about our tech support

February 15th, 2018

Here are few comments that our customers made on our tech support team:   “Expedeon technical support team has supported me in my experiments by helping and guiding me. The response time has been very very fast and the information provided very accurate!”   “Expedeon technical support staff was extremely helpful. Even though my inquiry was…

View

Strip test to diagnose Sickle Cell Anemia

February 5th, 2018

Introduction Sickle cell anemia (SCA)is caused by a mutation of the beta globin gene. It affects about 300,000 newborns each year, and includes symptoms such as severe anemia, pain and decreased immune function. Individuals who are homozygous for the sickle cell gene mutation produce only HbS (genotype SS) are affected by the disease whereas heterozygotes,…

View

Streamline & expand your antibody manufacturing services

February 5th, 2018

Our Virtual Library concept can provide antibody manufacturers the unique opportunity to rapidly increase their antibody conjugate catalogue whilst avoiding the difficulties presented by the need to hold large inventories of items that do not have any sales history. How can our Virtual Library help you? A complete virtual model Avoid expensive inventory costs with…

View

SunScript™

February 2nd, 2018

SunScript™ Reverse Transcriptase Basis of the Reverse Transcription Reaction SunScript™ Reverse Transcriptase allows reaction temperatures up to 85°C 100 ng of total RNA from HeLa cells were reverse transcribed at the indicated temperatures for 60 min with SunScript™ Reverse Transcriptases and Oligo(dT)18 primer, followed by inactivation at 95°C for 10 min. Presence of GAPDH cDNA…

View

TruePrime™

February 2nd, 2018

How does TruePrime™ technology work?   TruePrime™: absence of primer artefacts 1 pg of human genomic DNA (~ 1/6 of the content of one human/mammalian cell) has been amplified using either TruePrime™ (TthPrimPol-based MDA) or random primed MDA reactions. Random primed reactions contain 20% of sequences that cannot be mapped to any organism in sequence…

View

Bis-Tris Gels Technical Information

February 1st, 2018

RunBlue® Bis-Tris Protein Gels have been developed by Expedeon™ to provide an alternative to NuPAGE® Bis-Tris gels. These protein gels run with the same voltages, in the same tanks, using the same MES/MOPS buffers as NuPAGE gels. RunBlue Bis-Tris gels achieve highly comparable results with exceptional reproducibility and resolution. Polyacrylamide gels are based on the…

View

Why Liquid Biopsies?

January 17th, 2018

Investors considering new developments in healthcare and science like ideas that have the potential to change existing treatment protocols or dramatically impact on an entire industry segment. Clayton Christensen of Harvard Business School coined the term “disruptive innovation” to refer to those innovations that “create new markets by discovering new categories of customers.” The liquid…

View

Wine sample microorganism populations amplification by TruePrime™ WGA kit for a Biome analysis with WineSeq technology

January 17th, 2018

  By Dr Alberto Acedo. Biome Makers Co founder http://www.biomemakers.com Introduction Biome Makers is a biotech startup specialized in microbiome understanding to provide comprehensive analysis for agricultural and industrial processes. Winemakers are faced with decisions throughout the winemaking process: from the growth conditions of their wines (choice of land, pruning, irrigation) to the time of…

View

SYGNIS presents feasibility data for cell free DNA amplification with TruePrime™ technology at two recent meetings

January 17th, 2018

SYGNIS has presented data showing feasibility of a TruePrime™-mediated amplification of so-called cell free DNA in the meeting “10th ISMRC International Symposium on Minimal Residual Cancer: Liquid Biopsy in Cancer Diagnostics and Treatment” in March in Hamburg, Germany, and at the at the CHI’s Fourth International Molecular Diagnostics Europe in Lisbon, Portugal. The data were…

View

5 Methods for Checking Single-Cell WGA Success and Genome Coverage before Sequencing

January 17th, 2018

Isolation of single-cells while retaining whole genome integrity is challenging, whether using fluorescence-activated cell sorting, microfluidics, laser-capture microdissection, micromanipulation, dilution, or other techniques. And whole genome amplification (WGA) can introduce amplification bias and artefacts due to the use of random primers in excess and/or PCR. Consequently single-cell WGA can fail or give poor genome coverage,…

View

Liquid Biopsy

January 17th, 2018

“Liquid biopsy” is a term coined to describe diagnostic procedures done on nucleic acids in the blood or in other bodily fluids (e.g. urine or cerebrospinal fluid (CSF)) of patients. Dying cells release DNA from their fragmented genomes into the bloodstream. This “cell-free” DNA can be broadly divided into two groups: The smaller size fragments…

View

Contamination – What to keep in mind when doing NGS sequencing

January 16th, 2018

The intention of this post is to bring a certain problem up onto the surface which is often ignored or even unknown: Contamination in the sequences acquired from Next Generation Sequencing. Contamination due to handling of the samples before sequencing Of course, all of us know that a clean lab is top priority for good…

View