Investors

GC-content – How to view this with TrueGC

The intention of this post is to help you using our GC-content tool TrueGC which will give you information about the GC-content of the NGS sequences you have.

What you need to use TrueGC

To use TrueGC, you will need sequences. You can use those sequences which were directly given by the next generation sequencing machine or sequences processed by you. The format in which the sequences should be is fastq or fasta, not in the zipped file format “gz”. You should use not more than 10,000 sequences (this is enough to get an overview of the GC-content, and you will not waste time uploading your sequences). You can use any organism!

The input screen

Here you can see our input screen. We need you to provide the desired data for our statistics and your email address to send you a notification when the tool has finished the analysis or some error occurred.

The results

You will get a pdf as result with several different pages:

GC-content per sequence

The first page gives you the GC-content in percent for every sequence you submitted. This is useful to get an idea of some sort of contamination with different GC-content sequences and easily spot which sequences are different, so you can take a closer look at them.

Histogram of GC-content

To give you a broader overview we have added a GC-content histogram, which should show you a distribution of GC-content counts around the expected GC-content of your genome or genome-part. In our example sequences it is a GC-content of 25%.

If you have any question about the science behind these tips, or any other question about contamination in sequencing, please contact us. If you have any other suggestions for blog post topics, please let us know.

Elroy Cuevas

The items was easy to find on the website. Order was smooth. Would have liked additional payment options on the BioSciences webpage.

Mari

TruePrime® Single Cell WGA Kit

TruePrime® Single Cell WGA Kit

I have used the kit for amplification of single cells extracted from bovine embryo. It worked well and the products were as expected. The products…

G Hassall

RunBlue™ Prestained Protein Markers

RunBlue™ Prestained Protein Markers

Prepared molecular weight markers for SDS-PAGE can be loaded directly without the need for sample preparation, and coloured bands are clearly visible. A good spread…

Andrea Rawlings

RunBlue™ Bis-Tris Protein Gels

RunBlue™ Bis-Tris Protein Gels

The RunBlue 4-12% gels give very good resolution of our protein bands. They are so convenient to use compared to casting our own gels, and…

Oded Babai

Latex Conjugation Kit – 400nm Blue

Latex Conjugation Kit – 400nm Blue

We used this product to screen antibodies for lateral flow assay, using these kits we were able to conjugate 10 antibodies very rapidly and easily,…

Pavel Shliaha

InstantBlue™ Protein Stain

InstantBlue™ Protein Stain

Great product! Compared to simply blue from Thermo, got the same sensitivity with no hassle of washing the gel or destaining! Highly recommended

Evgeny Mymrikov

InstantBlue™ Protein Stain

InstantBlue™ Protein Stain

InstantBlue is a good time-saving alternative to our self-made stain. Protein bands are visible already after 10 min incubation and after 1h it is complete.…

Hannah Valentino

InstantBlue™ Protein Stain

InstantBlue™ Protein Stain

This product stains the protein gels very quickly with no background coloration. It is a fantastic time saver!

Francesca Pisani

LumiBlue™ ECL Extreme

LumiBlue™ ECL Extreme

I have used the ECL Extreme for Western blot experiments. It is really fantastic: very sensitive detection (up to fgrams of protein of interest) and…