Lightning-Link® Rapid Biotin has been published in Nature!
October 24th, 2016
Fc Receptors (FcRs) are key molecules for the recognition and elimination of foreign antigens. Among members of this family, FcgRI is expressed on mono-myeloid cells, whose cross-link with immune complexes can initiate cellular responses and receptor/ligand internalization.
However, while the first event is well described, the cellular processes involving FcgRI/ligand endocytosis are less defined.
Therefore, the authors focus their attention on understanding how mechanisms involving phosphorylation and dephosphorylation events can contribute to regulate FcgRI/ligand endosome degradation. Specifically, they investigate how the Tyr phosphorylation state of the clathrin-mediated endocytic proteins is affected by FcgRI cross-linking with immune complexes.
Using applications such as Western blotting, immunoprecipitation the authors show increased of Tyr-phosphorylation in lysates of cells in which surface FcgRI cross-linked with specific mouse mAb, especially among proteins involved in clathrin-mediated endocytosis. In addition, the authors showed how the leukocyte immunoglobulin like receptor B4 (LILRB4) is capable of inhibiting the FcgRI immune response by dephosphorylating key signaling molecules.
The authors conclude that the leukocyte immunoglobulin like receptor B4 (LILRB4) can regulate the immune response by dephosphorylating Tyr proteins. The inhibition of Tyr-phosphorylation prevents FcgRI endocytosis and degradation, an event triggered by the cross-link between FcgRI and immune-complexes in mono-myeloid cells. Read more…
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Our Lightning-Link® Rapid Biotin Antibody Labeling kit enables direct labeling of antibodies, proteins, peptides or other biomolecules to biotin, allow conjugations to be set up in seconds, and used within minutes (less than 20 minutes). More benefits include:
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