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SunScript®

February 2nd, 2018

SunScript® Reverse Transcriptase Basis of the Reverse Transcription Reaction SunScript® Reverse Transcriptase allows reaction temperatures up to 85°C 100 ng of total RNA from HeLa cells were reverse transcribed at the indicated temperatures for 60 min with SunScript® Reverse Transcriptases and Oligo(dT)18 primer, followed by inactivation at 95°C for 10 min. Presence of GAPDH cDNA…

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How does TruePrime® technology work?

February 2nd, 2018

  TruePrime®: absence of primer artefacts 1 pg of human genomic DNA (~ 1/6 of the content of one human/mammalian cell) has been amplified using either TruePrime® (TthPrimPol-based MDA) or random primed MDA reactions. Random primed reactions contain 20% of sequences that cannot be mapped to any organism in sequence databases. TruePrime® is insensitive to…

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Bis-Tris Gels Technical Information

February 1st, 2018

RunBlue® Bis-Tris Protein Gels have been developed by Expedeon™ to provide an alternative to NuPAGE® Bis-Tris gels. These protein gels run with the same voltages, in the same tanks, using the same MES/MOPS buffers as NuPAGE gels. RunBlue Bis-Tris gels achieve highly comparable results with exceptional reproducibility and resolution. Polyacrylamide gels are based on the…

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Why Liquid Biopsies?

January 17th, 2018

Investors considering new developments in healthcare and science like ideas that have the potential to change existing treatment protocols or dramatically impact on an entire industry segment. Clayton Christensen of Harvard Business School coined the term “disruptive innovation” to refer to those innovations that “create new markets by discovering new categories of customers.” The liquid…

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Wine sample microorganism populations amplification by TruePrime® WGA kit for a Biome analysis with WineSeq technology

January 17th, 2018

  By Dr Alberto Acedo. Biome Makers Co founder http://www.biomemakers.com Introduction Biome Makers is a biotech startup specialized in microbiome understanding to provide comprehensive analysis for agricultural and industrial processes. Winemakers are faced with decisions throughout the winemaking process: from the growth conditions of their wines (choice of land, pruning, irrigation) to the time of…

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SYGNIS presents feasibility data for cell free DNA amplification with TruePrime® technology at two recent meetings

January 17th, 2018

SYGNIS has presented data showing feasibility of a TruePrime®-mediated amplification of so-called cell free DNA in the meeting “10th ISMRC International Symposium on Minimal Residual Cancer: Liquid Biopsy in Cancer Diagnostics and Treatment” in March in Hamburg, Germany, and at the at the CHI’s Fourth International Molecular Diagnostics Europe in Lisbon, Portugal. The data were…

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5 Methods for Checking Single-Cell WGA Success and Genome Coverage before Sequencing

January 17th, 2018

Isolation of single-cells while retaining whole genome integrity is challenging, whether using fluorescence-activated cell sorting, microfluidics, laser-capture microdissection, micromanipulation, dilution, or other techniques. And whole genome amplification (WGA) can introduce amplification bias and artefacts due to the use of random primers in excess and/or PCR. Consequently single-cell WGA can fail or give poor genome coverage,…

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Liquid Biopsy

January 17th, 2018

“Liquid biopsy” is a term coined to describe diagnostic procedures done on nucleic acids in the blood or in other bodily fluids (e.g. urine or cerebrospinal fluid (CSF)) of patients. Dying cells release DNA from their fragmented genomes into the bloodstream. This “cell-free” DNA can be broadly divided into two groups: The smaller size fragments…

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Contamination – What to keep in mind when doing NGS sequencing

January 16th, 2018

The intention of this post is to bring a certain problem up onto the surface which is often ignored or even unknown: Contamination in the sequences acquired from Next Generation Sequencing. Contamination due to handling of the samples before sequencing Of course, all of us know that a clean lab is top priority for good…

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TruePrime® – Nature Communications paper published

January 16th, 2018

We are very proud to announce that the renowned scientific journal Nature Communications has published a paper on our TruePrime® method for single cell whole genome amplification, titled “TruePrime® is a novel method for whole-genome amplification from single cells based on TthPrimPol”. We hope that others can benefit from the knowledge shared by our expert…

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Evenness of coverage – An important alignment feature

January 16th, 2018

The intention of this post is to give a short introduction into the topic of evenness of coverage, which is an important feature of a sequence alignment. Evenness – Why should I check this? In NGS (whole genome sequencing/whole exome sequencing/amplicon sequencing) you normally get reads or sequences from a sequencing machine or company and…

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Evenness – How to view this with TruePlane

January 16th, 2018

The intention of this post is to help you using our evenness tool TruePlane which will give you an idea about the evenness of the NGS sequences you have. What you need to use TruePlane To use TruePlane, you will need sequences. You can use those sequences which were directly given by the next generation…

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Capal

TruePrime® Single Cell WGA Kit

TruePrime® Single Cell WGA Kit

I've used this kit for amplification of DNA from single flow-sorted plant chromosomes. Performance was exactly as desribed, on average I've obtained more than microgram…

Andrea Rawlings

Lightning-Link® Rapid Biotin - Type A

Lightning-Link® Rapid Biotin - Type A

This product worked very effectively and produced a good level of biotinylation. The instructions were easy to follow, and the reaction was fast and reliable.

Boyan Grigorov

InstantBlue™ Protein Stain

InstantBlue™ Protein Stain

We have tried InstantBlue in order to have a rapid result and, yes, it works for 15 minutes as mentioned in the protocol. Before, students…

Alistair

Lightning-Link® R-Phycoerythrin (R-PE)

Lightning-Link® R-Phycoerythrin (R-PE)

The procedure was very simple and gave excellent results

Amy

Lightning-Link® Rapid FluoProbes647H

Lightning-Link® Rapid FluoProbes647H

This kit was very easy to use, and the process of labeling my antibodies is quick. Because I used commercially purchased antibodies, I first had…

Andrea Rawlings

RunBlue™ Bis-Tris Protein Gels

RunBlue™ Bis-Tris Protein Gels

The RunBlue 4-12% gels give very good resolution of our protein bands. They are so convenient to use compared to casting our own gels, and…

Andrea Rawlings

AminTRAP HIS Prepacked Columns

AminTRAP HIS Prepacked Columns

We use the AminTrap nickel columns for purifying 6xHis tagged proteins using an automated purification system. The columns are completely compatible to our system and…

Adel Serwy

excellent,good knowledge.

Andreas

InstantBlue™ Protein Stain

InstantBlue™ Protein Stain

InstantBlue is more sensitive and faster than Coomassie blue stain. You could see clear bands after 10 minutes of incubation. Overnight incubation with InstantBlue is…

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