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Alexa Fluor® 488 antibody labeling kit

September 28th, 2017

Label your primary antibody in under 20 minutes The Expedeon Lightning-Link® Rapid Alexa Fluor® 488 antibody labeling kit enables the direct labeling of your primary antibody to Alexa Fluor® 488 with only 30 seconds hands-on time and with no loss of precious antibody. Unique benefits include: Direct conjugation of antibodies, proteins and peptides – no…

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Easy to use Biotin kits

September 28th, 2017

Minimize assay background with Expedeon easy to use biotin kits Our Lightning-Link® Rapid Biotinylation Kit (Type A & Type B) enables the direct biotinylation of antibodies, proteins and peptides, or any other biomolecule with an available amine group, with 100% recovery of materials. The kit requires only 30 seconds hands-on time and the conjugates will be…

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Microbiology & Immunology 2017 Virtual Event

September 11th, 2017

Expedeon is exhibiting at the Microbiology & Immunology 2017 Virtual Conference! Join us in bringing the Microbiology research community together online in discovering new concepts, tools and techniques to apply to ongoing research and diagnostics. This conference is free to attend. Microbiology and Immunology cover an immense scope, encompassing various industries and research areas including…

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Antibody purification kit selection

August 25th, 2017

Expedeon specializes in easy to use bioconjugation kits which enable the direct labeling of antibodies or proteins with enzymes, fluorescent labels, biotin, streptavidin, gold nanoparticles, latex beads or oligonucleotides. Unfortunately, many antibodies are provided in buffers which contain additives that are incompatible with labeling technologies, making purification a key consideration prior to carrying out any…

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Western blot protocol checklist

August 22nd, 2017

We have created a Western Blot Protocol Checklist – download it, print it and check off these tips from Innova scientists to maximize chances of great western blot results. From sample preparation, through transfer, all the way to detection, the checklist will help ensure that you haven’t missed any critical steps in the western blot…

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Direct vs indirect antibody labeling for flow cytometry

August 10th, 2017

What is meant by indirect detection? When secondary antibodies are used as the detection reagent in an assay, the detection process is referred to as being indirect. This is because two different antibody reagents are required for visualization of the target of interest – an antigen-specific primary antibody, and an anti-species secondary antibody. The secondary…

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The advantages of tandem dyes

August 9th, 2017

A significant number of laboratory applications rely on the use of fluorescently-labeled antibodies.

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5 tips for good bands with SDS-PAGE protein gels

August 9th, 2017

The aim of this short post is to provide a few easy tips that can help with getting consistently good bands when running homemade and precast SDS-PAGE protein gels. If you run protein gels you may already know some of these tips, but we hope you will find some useful information regardless. Consider heating and/or…

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Immunofluorescence, Immunocytochemistry and Immunohistochemistry – what’s the difference?

August 7th, 2017

The terms immunofluorescence, immunocytochemistry and immunohistochemistry are often used interchangeably. They are however clearly distinct from one another, and it is important that they are used appropriately to avoid confusion. The prefix, “immuno”, refers specifically to the binding of an antibody to an antigen, while the suffix confers different meanings: Immunofluorescence (IF) is a detection…

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Western blot resources

August 7th, 2017

Our Lightning-Link®, InnovaCoat® GOLD, and Expedeon products have been specifically designed to deliver a faster, more reliable Western blot. The Lightning-Link® antibody conjugation kits and the InnovaCoat® GOLD nanoparticles conjugation kits help researchers to avoid time-consuming secondary antibody incubation steps which may introduce background staining. Both product ranges take advantage of our proprietary conjugation technology…

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Passive versus covalent conjugation of antibodies to gold nanoparticles

July 3rd, 2017

Antibodies can be attached to gold nanoparticles by a variety of methods. These include passive adsorption, covalent attachment to surface-functionalized nanoparticles, and attachment to nanoparticles which have been pre-coated with a binding reagent such as streptavidin, biotin or Protein A/G. Passive adsorption Passive adsorption relies primarily on hydrophobic attractions and electrostatic interactions between the antibody…

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Enzyme labels for immunodetection

June 23rd, 2017

One of the most popular methods of immunodetection is to pair an enzyme-labeled antibody with an appropriate substrate. Enzymatic conversion of the substrate produces a colored, fluorescent or chemiluminescent readout, which can be used to determine the quantity of antigen present in the test sample. The most commonly used immunodetection enzymes are Horseradish Peroxidase (HRP),…

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