Five Tips for Good Bands with SDS PAGE Protein Gels

August 9th, 2017

The aim of this short post is to provide a few easy tips that can help with getting consistently good bands when running homemade and precast SDS PAGE protein gels. If you run protein gels you may already know some of these tips, but we hope you will find some useful information regardless. Consider Heating…


Immunofluorescence, Immunocytochemistry and Immunohistochemistry – what’s the difference?

August 7th, 2017

The terms immunofluorescence, immunocytochemistry and immunohistochemistry are often used interchangeably. They are however clearly distinct from one another, and it is important that they are used appropriately to avoid confusion. The prefix, “immuno”, refers specifically to the binding of an antibody to an antigen, while the suffix confers different meanings: Immunofluorescence (IF) is a detection…


Western blot resources

August 7th, 2017

Our Lightning-Link®, InnovaCoat® GOLD, and Expedeon products have been specifically designed to deliver a faster, more reliable Western blot. The Lightning-Link® antibody conjugation kits and the InnovaCoat® GOLD nanoparticles conjugation kits help researchers to avoid time-consuming secondary antibody incubation steps which may introduce background staining. Both product ranges take advantage of our proprietary conjugation technology…


Passive versus covalent conjugation of antibodies to gold nanoparticles

July 3rd, 2017

Antibodies can be attached to gold nanoparticles by a variety of methods. These include passive adsorption, covalent attachment to surface-functionalized nanoparticles, and attachment to nanoparticles which have been pre-coated with a binding reagent such as streptavidin, biotin or Protein A/G. Passive adsorption Passive adsorption relies primarily on hydrophobic attractions and electrostatic interactions between the antibody…


Enzyme labels for immunodetection

June 23rd, 2017

One of the most popular methods of immunodetection is to pair an enzyme-labeled antibody with an appropriate substrate. Enzymatic conversion of the substrate produces a colored, fluorescent or chemiluminescent readout, which can be used to determine the quantity of antigen present in the test sample. The most commonly used immunodetection enzymes are Horseradish Peroxidase (HRP),…


AACC – FREE Exhibit Hall Guest Pass

June 13th, 2017

FREE Exhibit Hall Guest Pass 69th AACC Annual Scientific Meeting & Clinical Lab Expo We are attending the 69th AACC Annual Scientific Meeting & Clinical Lab Expo this July in San Diego. As you might be aware, AACC is an opportunity to connect with global leaders in clinical chemistry, molecular diagnostics, mass spectrometry, translational medicine, lab…


Flow cytometry for CD4+ and CD8+ T cells

May 26th, 2017

What are CD antigens? Cluster of Differentiation (CD) molecules are found on the surface of leukocytes (white blood cells). They are useful for the identification of different cell types, and mediate a wide variety of immune functions. To date, over 350 CD molecules have been characterized through a series of international forums known as the…

10 most popular questions on Immuno-PCR webinar

10 most popular questions from the Immuno-PCR webinar

March 30th, 2017

Here are the 10 most popular questions from the Immuno-PCR webinar 1. How can I confirm that my antibody-oligo conjugation has been successful? The best way of doing this is to use the conjugate in your downstream application, however it’s also possible to analyze your antibody-oligo conjugate by gel electrophoresis. By running a small amount…

Lightning-Link® Fluorescein

New Reference – Lightning-Link® Fluorescein

February 7th, 2017

A Role for Glycans in Bla g 2 Cockroach Allergen-Induced Allergic Responses Introduction Cockroaches are a source of allergens that are capable of driving allergic respiratory symptoms, and which can lead to an increased risk for asthma. Immune responses triggered by cockroach allergens are most common among low-income urban populations in the United States, and…

10 most popular questions from LFA webinar

10 most popular questions from the LFA webinar

January 26th, 2017

1. What’s the best size of gold nanoparticle to use for a lateral flow immunoassay? 40nm is the most widely used, however it’s definitely possible to use other sizes of nanoparticle for lateral flow. 2. How many lateral flow immunoassay strips can I prepare from a vial of Expedeon’ latex beads? This varies from one…

InnovaCoat® GOLD nanoparticles reference

New Reference – InnovaCoat® GOLD nanoparticles

December 6th, 2016

Our InnovaCoat® GOLD nanoparticles and conjugation kits have been recently published! Introduction Vitamin B12 is a water-soluble vitamin, necessary for a variety of cellular functions such as DNA synthesis and normal blood formation. Low levels of vitamin B12 are mainly caused by low consumption of meat, fish and dairy products. Commercially available kits for diagnostic…

InnovaCoat® GOLD

Using InnovaCoat® GOLD in your assay – references

November 28th, 2016

The use of ultra-pure gold nanoparticles such as InnovaCoat® GOLD conjugation kits produces highly stable conjugates that can be used in a variety of scientific applications. Take a look at some of the published research in the table below that has used InnovaCoat® GOLD technology to produce antibody:nanoparticle conjugates for use in a variety of scientific applications……


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