TruePrime – Nature Communications paper published

We are very proud to announce that the renowned scientific journal Nature Communications has published a paper on our TruePrime method for single cell whole genome amplification, titled “TruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol”. We hope that others can benefit from the knowledge shared by our expert team in this paper, and we look forward to both seeing and being a key part of further advances in this field. The review process helps validate the technology used in our TruePrime amplification kits, and as you may expect we’re very excited about it!

You can read the full paper here:

The abstract is below for your convenience. We’d be glad to help with any questions you might have about the publication, please contact us. You could also subscribe to our newsletter if you’d like to keep informed of product news and new blog posts.

Abstract published in Nature Communications:

“Sequencing of a single cell genome requires DNA amplification, a process prone to introducing bias and errors into the amplified genome. Here we introduce a novel multiple displacement amplification (MDA) method based in the unique DNA primase features of Thermus thermophilus (Tth) PrimPol. TthPrimPol displays a potent primase activity preferring dNTPs as substrates unlike conventional primases. A combination of TthPrimPol’s unique ability to synthesize DNA-primers with the highly processive Phi29 DNA polymerase (Φ29DNApol) enables near complete whole genome amplification from single cells. This novel method demonstrates superior breadth and evenness of genome coverage, high reproducibility, excellent single nucleotide variant (SNV) detection rates with low allelic dropout (ADO) and low chimera formation as exemplified by sequencing Hek293 cells. Moreover, copy number variant (CNV) calling yields superior results compared to random primer-based MDA methods. The advantages of this method, that we named TruePrimeTM, promise to facilitate and improve single cell genomic analysis.”