Antibody–Oligo Micro-optimization Service

Optimizing your antibody–oligonucleotide conjugates can be time-consuming and expensive, especially when using traditional multistep conjugation techniques.

Our Antibody–Oligonucleotide Micro-optimization Service is uniquely performed by our experienced scientists here at Expedeon. We use unique conjugation conditions that can enhance the performance of even the most temperamental of antibodies.

Benefits of this Service Include:

  • A choice of three different scales of conjugate 50ug,100ug, and 500ug
  • Antibody clean-up prior to conjugation
  • Level of conjugation controlled by a variety of parameters unique to Expedeon
  • Removal of unbound oligo
  • Evidence of successful conjugation provided
  • Scalable
  • Fast turnaround time
  • Reproducible
  • Full technical support
  • Applicable to other proteins.

What’s Included?

  • Six different conjugates at the chosen scale, including: three different chemistries, different antibody–oligo ratios.
  • Antibody clean-up prior to conjugation
  • Removal of unbound oligo postconjugation
  • Proof of successful conjugation in the form of a QC gel.

We can make your life even easier by supplying your oligo and antibody and we can even purify your antibody if required.

Example Data

Altering conjugation conditions can generate conjugates with modified functional properties. The performance of an antibody–oligo conjugate is often related to the number of oligos bound to the antibody. There are two key parameters that control the number of oligos bound to each antibody; these are the number of ‘attachment sites’ on the antibody and the concentration of oligo in the conjugation reaction.

With our micro-optimization service, we exploit changes in both the number of antibody attachment sites and the concentration of oligo to generate a range of conjugates. The resulting conjugates differ in their level of bound oligo and functional properties with unique features that could possibly include decreased background, increased signal or improved sensitivity.

In the example shown below the micro-optimization service has been used to generate six different antibody/oligo conjugates. The conjugates have been analysed on a 4–12% acrylamide gel under reducing conditions. The gel has been stained for DNA using Sybr Gold DNA stain.

As can be seen from the figure below the six conjugates demonstrate a range of oligo labeling. The level of optimization can be tailored to individual needs and once chosen, all formations are reproducible and available in bulk quantities.

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