CaptSure™ Peptide Check&Go! – FAQS
1. What is the best storage condition for the kit?
The kit is shipped at ambient temperature and its components need different storage temperatures. Upon receipt, the plastic pot containing the strips should be stored at +4⁰C. The running buffer and the lyophilized GOLD vial should be stored at -20⁰C. Once reconstituted the GOLD conjugates are stable at +4⁰C for up to 1 month.
2. Can antibodies from different species be used?
Yes, the CaptSure Peptide Check&Go! strips have been tested with a variety of antibodies conjugated to CaptSure Peptide.
3. Why is the T-line faint after 10-minute incubation?
The CaptSure Peptide-tagged antibody concentration is outside of the dynamic range (0.1-1 μg/ml). We recommend reviewing dilutions calculation and repeat the assay.
4. Why is there no T-line after 10-minute incubation?
The CaptSure Peptide-tagged antibody conjugation has failed or is sub-optimal. Repeat the conjugation and test again.
5. Forgot to add 0.1% BSA to the running buffer, will this affect the assay?
The lack of BSA will not compromise the C-line or the T-line. However, the assay may run slower and GOLD may still be present at the top of the strips, below the wicking pad.
6. Can I use CaptSure Peptide Check&Go to confirm successful conjugation of my antibody to other labels?
CaptSurePeptide Check&Go! is only suitable for testing successful conjugation of your antibody to the LL-CaptSure Peptide. However we also provide a variety of other Check&Go! kits including: Biotin Check&Go! for verifying successful biotinylation, HRP Check&Go! for confirming conjugation of your antibody to HRP, Conjugate Check&Go! for testing the success of conjugation to coloured labels, such as fluorescent dyes and proteins, latex beads and gold nanoparticles and many more.