Conjugate Check&Go! FAQs
1. What is the best storage condition for the kit?
The kit is shipped at ambient temperature and its components need different storage temperature. Upon arrival, the plastic pot containing the strips, manufactured with molecular sieves integrated into the walls, must be kept at 4⁰C. The lyophilized control vial must be stored at -20⁰C; once reconstituted, the Rabbit IgG-GOLD conjugate is stable at 4⁰C for up to 2 weeks in the 1x Running Buffer + BSA. For long term storage, reconstitute the control vial in 40ul 1x Running Buffer (without BSA) and dilute further in 1x Running Buffer + BSA to run the LFA. Keep the 10x Running Buffer at 4⁰C.
2. Can antibodies from different species be used?
Yes, the Conjugate Check&Go! strips have been tested with antibodies from a variety of species including mouse, rabbit, goat and rat, providing they are conjugated to a colored label. The binding properties of Protein A and G, immobilized on the Test line of the strips, differ among species and subclasses of IgG. A low or no affinity for a specific IgG subclass may lead to low/no signal but the conjugate may be still fine.
3. Can conjugates from antibody fragments be checked?
The protein A and protein G immobilized on the Test line have a high affinity for the Fc region of IgG molecules, and therefore for the Fc fragments as well.
4. Can I used the Conjugate Check&Go! Kit to confirm successful conjugation of my antibody to any Lightning-Link label?
The Conjugate Check&Go kit is a quick immunochromatographic test designed to detect on the T line IgGs conjugated labels visible by eye, therefore the following labels are not compatibale with this kit: AMCA, Atto350, Atto390, Dylight® 405, Dylight® 800, Dylight® 755, Dylight® 700 and Dylight® 680, horseradish peroxidase, alkaline phosphatase, streptavidin or biotin.
5. How can I check my antibody conjugates if the label has a faint color?
If your antibody is conjugated to a faint label that is hardly detectable by eye, you can run your conjugate at higher concentration than the one recommended to obtain a visible line on the strips.
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