Investors

GTP Agarose FAQs

Q1. What is the best buffer to use?

There is no ‘right’ answer here. In the absence of any information on the binding requirements of the protein(s) of interest a good starting point is a buffer containing 20mM Hepes, 100-500mM NaCl (or KCl), 20mM MgCl2 and 1mM DTT, pH 7.5. Alternatively, try experimenting with several buffer conditions using small amounts of resin in 1.5ml tubes. After the wash step, elute with GTP or other competing ligand and analyse the elutedproteins by SDS-PAGE. The purification method can then be scaled up using the preferred buffer conditions.

Q2. Are there any other ways of eluting binding proteins from the GTP Agarose?

Yes. While GTP is the obvious choice, ligands that are structurally related to GTP may be used (e.g. GMP and guanosine) to elute a specific subset of the GTP-binding proteins. Drugs that are known to bind to GTP-binding proteins might also be used. If preservation of biological activity is not required, aliquots of resin may be boiled with SDS sample buffer prior to gel electrophoresis.

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Christopher Ball

Lightning-Link® Rapid Biotin - Type A

Lightning-Link® Rapid Biotin - Type A

Very good product worked every time!

Claire

InstantBlue™ Protein Stain

InstantBlue™ Protein Stain

Best protein stain ever used. Quick and easy to use. Even stains difficult proteins that normal coomassie stain does not.

Evgeny Mymrikov

InstantBlue™ Protein Stain

InstantBlue™ Protein Stain

InstantBlue is a good time-saving alternative to our self-made stain. Protein bands are visible already after 10 min incubation and after 1h it is complete.…

Elroy Cuevas

The items was easy to find on the website. Order was smooth. Would have liked additional payment options on the BioSciences webpage.

Will Michaels

RunBlue™ TEO-Tricine SDS Mini Gels

RunBlue™ TEO-Tricine SDS Mini Gels

Started using these gels because I needed to see both high and low mass proteins. They are far superior to my hand-cast tris-tricine (shagger) gels…

Will Michaels

RunBlue™ TEO-Tricine SDS Mini Gels

RunBlue™ TEO-Tricine SDS Mini Gels

Swapping to the TEO-Tricine gradient gels has allowed me to visualise the cleavage of a small peptide from a large MBP tag - whilst maintaining…

Pallab Maity

RunBlue™ TEO-Tricine SDS Gels

RunBlue™ TEO-Tricine SDS Gels

Our lab is using Expedeon gels and running buffers for last 6-7 years. These gels are great in quality with long self-life. Never heard from…

Andreas

InstantBlue™ Protein Stain

InstantBlue™ Protein Stain

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Stuart Wilson

Lightning-Link® Rapid Biotin - Type A

Lightning-Link® Rapid Biotin - Type A

It's so easy to biotinylate our antibody with this product.