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FAQs

Find answers to frequently asked questions about our product ranges in this section below.

ELISA‐ONE™ – FAQs

Frequently Asked Questions (FAQs) for ELISA‐ONE 1. Can I use a different ELISA plate to the one that is supplied? ELISA‐ONE microplates are specific to ELISA‐ONE and cannot be substituted with any other type of 96 well microplate. ELISA‐ONE microplates, which rely on our CaptSure™ technology, are optimized to provide an active surface for binding…

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Europium Conjugation Kit FAQs

1. What are the advantages of using our Europium Conjugation Kit? The Europium chelate microspheres have been specially treated to enhance the handling of the beads and permit easy covalent attachment of antibodies and other proteins. The conjugation reaction is initiated simply by adding a solution of the antibody to the lyophilized mixture; the hands…

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FFPE-FASP™ Protein Digestion Kit FAQ

What Does the FFPE-FASP Protein Digestion Kit Do? The FFPE-FASP Protein Digestion Kit: • Reverses protein crosslinking that originates from formalinfixation • Extracts the proteins from the tissue • Reduces the disulfide bonds • Depletes contaminants from any protein sample solution • Alkylates thiols • Digests proteins in a spin-filter “proteomic reactor” • Releases peptide…

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FlexLISA FAQs

1. What is different about the FlexLISA® kit? In a standard ELISA the capture antibody is bound directly to the surface of the well, whereas the 96 stripwell plate provided within the FlexLISA® kit is pre-coated with biotin which binds the capture antibody-streptavidin conjugate with a strong affinity. Therefore, much less capture antibody (40x less)…

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CaptSure™ DIY ELISA FAQ’S

1. Can I use a different ELISA plate to the one that is supplied? CaptSure Assay Plates are specific to CaptSure DIY ELISA and cannot be substituted with any other type of 96 well microplate. CaptSure Assay Plates, which rely on our CaptSure technology, are optimized to provide an active surface for binding the immunocomplex…

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GELFrEE 8100 FAQ’s

Basics What does the GELFrEE® 8100 Fractionation System do? The GELFrEE® 8100 System fractionates complex protein mixtures into liquid phase molecular fractions. It’s like being able to cut out any number of bands in a 1D and extract them into solution, but without slab gels and cutting, and with much higher recoveries. And, proteins are…

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GST Tag Check&Go! FAQS

Can I use re-use the GST Tag Check&Go! strips? No, as the GST Tag Check&Go! strips are designed for single use only. Can I use the GST Tag Check&Go! strips to quantify my GST tagged protein? The GST Tag Check&Go! strips provide a qualitative yes / no answer, although a semi-quantitative measurement of the abundance…

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GTP Agarose FAQs

Q1. What is the best buffer to use? There is no ‘right’ answer here. In the absence of any information on the binding requirements of the protein(s) of interest a good starting point is a buffer containing 20mM Hepes, 100-500mM NaCl (or KCl), 20mM MgCl2 and 1mM DTT, pH 7.5. Alternatively, try experimenting with several…

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His-Tag Check&Go! FAQs

Can I use re-use the His-Tag Check&Go! strips? No, as the His-Tag Check&Go! strips are designed for single use only. Can I use the His-Tag Check&Go! strips to quantify my His-tagged protein? The His-Tag Check&Go! strips provide a qualitative yes / no answer, although a semi-quantitative measurement of the abundance of protein in the sample…

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HRP Check&GO! FAQs

1.What are the best storage conditions for the kit? The kit is shipped at ambient temperature and its components need different storage temperatures. Upon receipt, the plastic pot containing the strips and the HRP detection solution should be stored at 4⁰C. The running buffer and the lyophilized control should be stored at -20⁰C. The lyophilized…

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InnovaCoat® GOLD FAQs

InnovaCoat® GOLD nanoparticles which covalently binds antibodies to form highly stable conjugates

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InstantBlue Protein Stain FAQs

What is the sensitivity of InstantBlue? It can detect as low as 5 ng of protein (BSA). Does InstantBlue has an upper limit of detection? The stain does have an upper limit dependent on the volume of the InstantBlue Stain used. The more InstantBlue, the more Coomassie dye that is free to bind to protein….

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