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Ponceau S FAQs

Is Ponceau S solution suitable for both nitrocellulose and PVDF membranes?

Yes, it is. Ponceau S solution has been tested on both nitrocellulose and PVDF membranes and it provided reproducible data as well as a high signal-to-noise ratio.

Is Ponceau S solution suitable with different blocking agents other than BSA?

Yes, it is. Ponceau S solution is suitable with the most common-use blocking agents, such as non-fat dry milk (3-5%), casein (1%) and fish skin gelatin (1%).

What is the recommended dilution for Ponceau S solution ?

Ponceau S solution is provided as a ready-to-use 1X solution

Can Ponceau S solution be used to detect proteins under both reducing and non-reducing conditions?

Yes, it can. Ponceau S solution has been tested on proteins run under both reducing and non-reducing conditions, with both conditions showing equal sensitivity of detection and reproducibility.

What is the limit of detection for Ponceau S solution?

Ponceau S solution can stain as little as 250ng of Bovine Serum Albumin on a nitrocellulose membrane.