Guides

Antibody Labeling Guides

Absorption Emission Table

Lightning-Link® is an innovative technology that enables direct labeling of antibodies, proteins, peptides or other biomolecules with only 30 seconds hands-on time.

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Antibody Manufacturing Guide

Expedeon provide antibody manufacturers with the unique opportunity to rapidly increase their antibody conjugates catalogue whilst avoiding the difficulties presented by the need to hold large inventories of items that do not have any sales history. Our Virtual Library concept can easily enhance your custom antibody conjugate offering as well as streamline commercial processes. Quick…

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Antibody Purification Guide

Download Your Copy Expedeon specializes in easy to use bioconjugation kits which enable the direct labeling of antibodies or proteins with enzymes, fluorescent labels, biotin, streptavidin, gold nanoparticles, latex beads or oligonucleotides. Unfortunately, many antibodies are provided in buffers which contain additives that are incompatible with labeling technologies, making purification a key consideration prior to…

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Guide to Labeling Your Primary Antibody

        Download a free copy! Antibodies are used to detect and quantify antigens in techniques such as flow cytometry, ELISA, western blotting, immunohistochemistry and lateral flow. The antibody that binds to the antigen is called a ‘primary antibody’ and it confers specificity to the assay. Most types of immunoassays also incorporate a…

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Simplifying Antibody Conjugation Process

Antibodies are widely employed in the quantification of antigens in complex biological samples. Using techniques such as Western blotting, ELISA, and immunohistochemistry researchers are able to measure a single antigen, or perhaps a limited number of antigens, in each sample. In the post-genomics era, advances in multiplex immunoassay technologies now allow scores or even hundreds…

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How and when to use the thiol conjugation approach: an introductory guide

A traditional method for linking proteins to molecules relies on the formation of a stable covalent bond between thiol (R-SH) and maleimide groups. When these functional groups are positioned on separate biomolecules (A & B) a hybrid conjugate AB (but not AA or BB) will form. Despite other systems of conjugation being available (for instance,…

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A-Z Guide to Fluorochromes

Download a Free Copy Lightning-Link® fluorescent antibody and protein labeling kits allow you to covalently label any antibody in under 20 minutes with only 30 seconds hands-on time. We currently have over 35 labels in our range of fluorescent dyes. This guide provides a general overview of each fluorochrome in our range including their properties,…

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Application Guides

The Advantages of Tandem Dyes

A significant number of laboratory applications rely on the use of fluorescently-labeled antibodies.

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A Beginner’s Guide to Flow Cytometry

Flow Cytometry is a widely used method for cell analysis which, for the novice, can appear daunting due to the complexity of the experimental approach and data analysis.

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Development of an Immuno-PCR Assay

        Discover how the Immuno-PCR assay combines the specificity of an ELISA with the signal amplification of a PCR. This guide will provide you with a good overview of this application. It covers the following: What is Immuno-PCR? Development of an Immuno-PCR assay to quantify Prostate Specific Antigen (PSA) The main steps…

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ELISA Principles, Formats & Streamlining

A short overview of the principles and different formats of ELISA is provided on this webpage. If you want to find out more, download our complete guide by filling out the form on the right-hand side. What is ELISA? ELISA (Enzyme-Linked Immunosorbent Assay) is a plate-based technique invented in the 1970s and evolved from the…

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Fast, Simple and Cost Effective Immunoassay Development

SPARCL (Spatial Proximity Analyte Reagent Capture Luminescence) is a novel homogeneous proximity assay technology utilizing flash chemiluminescence detection without solid support or wash steps. This format allows for rapid immunoassay development, in as little as 2 days. The homogenous format promotes fast antibody to antigen binding kinetics, allowing 30 minute incubation times. With the SPARCL…

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Guide to Lateral Flow Immunoassays

        The evolution of lateral flow immunoassays Components Process options Assay formats Advantages & disadvantages of lateral flow immunoassays Nanoparticles for lateral flow immunoassays

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Guide to Western blotting

        Download a free copy The Western blot technique requires samples to be resolved on the basis of size through Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE), following which they are transferred to, and immobilized on, a membrane prior to antibody-based detection. Find out how we can help you with your Western…

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Immunohistochemistry

Free copy! Immunohistochemistry (IHC), or immunohistochemical staining, is a technique that employs antibodies to detect antigens in cells within a small section of tissue. The application is used to locate specific antigens in tissue sections with labeled antibodies, based on antigen:antibody interactions. Immune reactive products can be visualized by a marker. These markers  include fluorescent…

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Thiol conjugation reactions and creating and quantifying protein thiols

Thiol conjugation reactions To help you better understand if the thiol-maleimide approach is the right one for you, we have published this new guide on thiol conjugation reactions.  Download this guide, if you want to know more about: The thiol-maleimide approach and its advantages The difference between thiol groups and disulfides and how thiol groups…

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Enzyme Guides

Enzyme Conjugates for IVD Applications

Enzyme-antibody conjugates are key components of a variety of IVD products, including enzymelinked immunosorbent assay (ELISA) kits, lateral flow immunochromatographic devices, and kits based on immunohistochemistry and immunoblotting. The analyte of interest is detected by the antibody component of the conjugate, and measurability is provided by the enzyme. The assay signal is either proportional or…

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Enzyme Unit Definitions and Assay Design

Download a Free Copy This guide explains key concepts such as ‘enzyme units’ and ‘enzyme activity’ in simple terms and discusses enzyme assay design and the importance of operating in the ‘linear range’. There are also some tips on setting up assay controls and subtracting blanks. This guide covers: Definitions of enzyme units What is…

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PiColorLock™ detection reagent

The phosphate detection reagent, PiColorLock™, changes color in the presence of inorganic phosphate (Pi) and this property can be exploited to measure the activity of any enzyme that generates Pi. The reagent is applicable to a large number of enzymes including ATPases, GTPases, phosphatases, heat-shock proteins and DNA unwinding proteins. The unique formulation of PiColorLock™…

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Gold nanoparticles

Gold Nanoparticles from Expedeon

Each antibody is unique and it is important to have a range of options for making nanoparticle conjugates to achieve the best possible performance in diagnostic assays. Antibodies may be attached to coated gold nanoparticles using simple covalent conjugation technologies or with traditional passive techniques.

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Posters

Tech note – Wine sample microorganism populations amplification by TruePrime®

This application note describes how microorganism populations can be amplified using TruePrime®

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4.17

Capal

TruePrime® Single Cell WGA Kit

TruePrime® Single Cell WGA Kit

I've used this kit for amplification of DNA from single flow-sorted plant chromosomes. Performance was exactly as desribed, on average I've obtained more than microgram…

Andrea Rawlings

Lightning-Link® Rapid Biotin - Type A

Lightning-Link® Rapid Biotin - Type A

This product worked very effectively and produced a good level of biotinylation. The instructions were easy to follow, and the reaction was fast and reliable.

Boyan Grigorov

InstantBlue™ Protein Stain

InstantBlue™ Protein Stain

We have tried InstantBlue in order to have a rapid result and, yes, it works for 15 minutes as mentioned in the protocol. Before, students…

Alistair

Lightning-Link® R-Phycoerythrin (R-PE)

Lightning-Link® R-Phycoerythrin (R-PE)

The procedure was very simple and gave excellent results

Amy

Lightning-Link® Rapid FluoProbes647H

Lightning-Link® Rapid FluoProbes647H

This kit was very easy to use, and the process of labeling my antibodies is quick. Because I used commercially purchased antibodies, I first had…

Andrea Rawlings

RunBlue™ Bis-Tris Protein Gels

RunBlue™ Bis-Tris Protein Gels

The RunBlue 4-12% gels give very good resolution of our protein bands. They are so convenient to use compared to casting our own gels, and…

Andrea Rawlings

AminTRAP HIS Prepacked Columns

AminTRAP HIS Prepacked Columns

We use the AminTrap nickel columns for purifying 6xHis tagged proteins using an automated purification system. The columns are completely compatible to our system and…

Adel Serwy

excellent,good knowledge.

Andreas

InstantBlue™ Protein Stain

InstantBlue™ Protein Stain

InstantBlue is more sensitive and faster than Coomassie blue stain. You could see clear bands after 10 minutes of incubation. Overnight incubation with InstantBlue is…

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