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Antibody Labeling Guides

The Biotin Streptavidin Interaction

Streptavidin and Biotin form the strongest noncovalent biological interaction The affinity of streptavidin for biotin is the strongest noncovalent biological interaction known, with a dissociation constant (Kd) in the femtomolar range. Each streptavidin monomer can bind one biotin molecule, allowing a streptavidin protein to maximally bind four biotins. The Streptavidin-Biotin Interaction In addition to the…

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Antibody Manufacturing Guide

  Expedeon provide antibody manufacturers the unique opportunity to rapidly increase their antibody conjugate catalogue whilst avoiding the difficulties presented by the need to hold large inventories of items that do not have any sales history. Our Virtual Library concept can easily enhance your custom antibody conjugate offering as well as streamline commercial processes. Quick…

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A-Z Guide to Fluorochromes

        Download a Free Copy Lightning-Link® fluorescent antibody and protein labeling kits allow you to covalently label any antibody in under 20 minutes with only 30 seconds hands-on time. We currently have over 35 label in our range of fluorescent dyes. This guide provides a general overview of each fluorochrome in our…

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Absorption-Emission Table

Lightning-Link® is an innovative technology that enables direct labeling of antibodies, proteins, peptides or other biomolecules with only 30 seconds hands-on time.

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Simplifying Antibody Conjugation Process

Antibodies are widely employed in the quantification of antigens in complex biological samples. Using techniques such as Western blotting, ELISA, and immunohistochemistry researchers are able to measure a single antigen, or perhaps a limited number of antigens, in each sample. In the post-genomics era, advances in multiplex immunoassay technologies now allow scores or even hundreds…

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Antibody Purification Guide

        Download your copy Expedeon specializes in easy to use bioconjugation kits which enable the direct labeling of antibodies or proteins with enzymes, fluorescent labels, biotin, streptavidin, gold nanoparticles, latex beads or oligonucleotides. Unfortunately, many antibodies are provided in buffers which contain additives that are incompatible with labeling technologies, making puri cation…

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Guide to labeling your primary antibody

        Download a free copy! Antibodies are used to detect and quantify antigens in techniques such as flow cytometry, ELISA, western blotting, immunohistochemistry and lateral flow. The antibody that binds to the antigen is called a ‘primary antibody’ and it confers specificity to the assay. Most types of immunoassays also incorporate a…

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How and when to use the thiol conjugation approach: an introductory guide

A traditional method for linking proteins to molecules relies on the formation of a stable covalent bond between thiol (R-SH) and maleimide groups. When these functional groups are positioned on separate biomolecules (A & B) a hybrid conjugate AB (but not AA or BB) will form. Despite other systems of conjugation being available (for instance,…

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