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Application Guides
ELISA Principles, Formats & Streamlining
Download a Free Copy This guide provides a comprehensive overview of ELISA, describing the principles and different formats of this highly sensitive and flexible application. It also discusses methods of optimizing and streamlining your ELISA. Find out more: What is an ELISA? What are the advantages of an ELISA? Different…
Thiol conjugation reactions and creating and quantifying protein thiols
Thiol conjugation reactions To help you better understand if the thiol-maleimide approach is the right one for you, we have recently published our new guide to thiol conjugation reactions, download now for free. Download this guide, if you want to know more about: The thiol-maleimide approach and its advantages The difference between thiol groups and…
Guide to Lateral Flow Immunoassays
The evolution of lateral flow immunoassays Components Process options Assay formats Advantages & disadvantages of lateral flow immunoassays Nanoparticles for lateral flow immunoassays
Guide to Western blotting
Download a free copy The Western blot technique requires samples to be resolved on the basis of size through Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE), following which they are transferred to, and immobilized on, a membrane prior to antibody-based detection. Find out how we can help you with your Western…
Development of an Immuno-PCR Assay
Discover how the Immuno-PCR assay combines the specificity of an ELISA with the signal amplification of a PCR. This guide will provide you with a good overview of this application. It covers the following: What is Immuno-PCR? Development of an Immuno-PCR assay to quantify Prostate Specific Antigen (PSA) The main steps…
Immunohistochemistry
Immunohistochemistry (IHC), or immunohistochemical staining, is a technique which employs antibodies to detect antigens in cells within a tissue section. This application is used to locate specific antigens in tissue sections with labeled antibodies based on antigen-antibody interactions. The immune reactive products can be visualized by a marker including fluorescent dyes,…
A Beginner’s Guide to Flow Cytometry
Flow Cytometry is a widely used method for cell analysis which, for the novice, can appear daunting due to the complexity of the experimental approach and data analysis.
Fast, Simple and Cost Effective Immunoassay Development
SPARCL (Spatial Proximity Analyte Reagent Capture Luminescence) is a novel homogeneous proximity assay technology utilizing flash chemiluminescence detection without solid support or wash steps. This format allows for rapid immunoassay development, in as little as 2 days. The homogenous format promotes fast antibody to antigen binding kinetics, allowing 30 minute incubation times. With the SPARCL…
The advantages of tandem dyes
A significant number of laboratory applications rely on the use of fluorescently-labeled antibodies.
