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Application Guides
ELISA Principles, Formats & Streamlining
A short overview of the principles and different formats of ELISA is provided on this webpage. If you want to find out more, download our complete guide by filling out the form on the right-hand side. What is ELISA? ELISA (Enzyme-Linked Immunosorbent Assay) is a plate-based technique invented in the 1970s and evolved from the…
Thiol conjugation reactions and creating and quantifying protein thiols
Thiol conjugation reactions To help you better understand if the thiol-maleimide approach is the right one for you, we have published this new guide on thiol conjugation reactions. Download this guide, if you want to know more about: The thiol-maleimide approach and its advantages The difference between thiol groups and disulfides and how thiol groups…
Guide to Lateral Flow Immunoassays
The main application to drive the development of early lateral flow immunoassays was the human pregnancy test, which relied on the detection of human Chorionic Gonadotropin (hCG) in urine. hCG is a hormone which is secreted during the early stages of pregnancy, and plays an essential role in early pregnancy survival; its presence in urine…
Guide to Western blotting
Download a free copy The Western blot technique requires samples to be resolved on the basis of size through Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE), following which they are transferred to, and immobilized on, a membrane prior to antibody-based detection. Find out how we can help you with your Western…
Development of an Immuno-PCR Assay
Discover how the Immuno-PCR assay combines the specificity of an ELISA with the signal amplification of a PCR. This guide will provide you with a good overview of this application. It covers the following: What is Immuno-PCR? Development of an Immuno-PCR assay to quantify Prostate Specific Antigen (PSA) The main steps…
Immunohistochemistry
Free copy! Immunohistochemistry (IHC), or immunohistochemical staining, is a technique that employs antibodies to detect antigens in cells within a small section of tissue. The application is used to locate specific antigens in tissue sections with labeled antibodies, based on antigen:antibody interactions. Immune reactive products can be visualized by a marker. These markers include fluorescent…
A Beginner’s Guide to Flow Cytometry
Flow Cytometry is a widely used method for cell analysis which, for the novice, can appear daunting due to the complexity of the experimental approach and data analysis.
Fast, Simple and Cost Effective Immunoassay Development
SPARCL (Spatial Proximity Analyte Reagent Capture Luminescence) is a novel homogeneous proximity assay technology utilizing flash chemiluminescence detection without solid support or wash steps. This format allows for rapid immunoassay development, in as little as 2 days. The homogenous format promotes fast antibody to antigen binding kinetics, allowing 30 minute incubation times. With the SPARCL…
The Advantages of Tandem Dyes
A significant number of laboratory applications rely on the use of fluorescently-labeled antibodies.
