Guide to Western blotting

 

 

 

 

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The Western blot technique requires samples to be resolved on the basis of size through Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE), following which they are transferred to, and immobilized on, a membrane prior to antibody-based detection.

Find out how we can help you with your Western blotting, from sample preparation to visualization. Find in this guide:

  • The main stages of a Western blot: Sample preparation, Gel electrophoresis, Membrane transfer, and Immunoblotting
  • Use of directly-labeled primary antibodies for Western blotting
  • Methods of protein detection: Colorimetric detection, Fluorometric detection, Chemiluminescence, and gold nanoparticles

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