August 23rd, 2017
Immunohistochemistry (IHC), or immunohistochemical staining, is a technique which employs antibodies to detect antigens in cells within a tissue section.
This application is used to locate specific antigens in tissue sections with labeled antibodies based on antigen-antibody interactions.
The immune reactive products can be visualized by a marker including fluorescent dyes, enzymes, radioactive elements or colloidal gold.
This comprehensive guide covers:
- Principles of Immunohistochemistry (IHC) & its common uses
- Antigen retrieval
- Indirect vs. Direct Detection Methods
- And much more
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