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Immunohistochemistry (IHC), or immunohistochemical staining, is a technique that employs antibodies to detect antigens in cells within a small section of tissue.

The application is used to locate specific antigens in tissue sections with labeled antibodies, based on antigen:antibody interactions.

Immune reactive products can be visualized by a marker. These markers  include fluorescent dyes, enzymes, radioactive elements or colloidal gold.

This comprehensive guide covers:

  1. Principles of Immunohistochemistry (IHC) & its common uses
  2. Fixation
  3. Antigen retrieval
  4. Antibodies
  5. Indirect vs. Direct Detection Methods
  6. And much more…


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