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Immunohistochemistry

 

 

 

 

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Immunohistochemistry (IHC), or immunohistochemical staining, is a technique which employs antibodies to detect antigens in cells within a tissue section.

This application is used to locate specific antigens in tissue sections with labeled antibodies based on antigen-antibody interactions.

The immune reactive products can be visualized by a marker including fluorescent dyes, enzymes, radioactive elements or colloidal gold.

This comprehensive guide covers:

  1. Principles of Immunohistochemistry (IHC) & its common uses
  2. Fixation
  3. Antigen retrieval
  4. Antibodies
  5. Indirect vs. Direct Detection Methods
  6. And much more

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