Thiol conjugation reactions and creating and quantifying protein thiols

Thiol conjugation reactions

To help you better understand if the thiol-maleimide approach is the right one for you, we have published this new guide on thiol conjugation reactions.

 Download this guide, if you want to know more about:

  • The thiol-maleimide approach and its advantages
  • The difference between thiol groups and disulfides and how thiol groups are generated
  • How to find out if your sample contains free thiol groups
  • Why thiol reducing agents such as Mercaptoethanol, DTT and TCEP are not interchangeable and how to know which one to choose in your application
  • How to generate maleimide groups in your sample
  • How to take advantage of a range of maleimide-activated labels in your assay
  • How to measure the presence of maleimide groups
  • How optimizing the number of reactive groups can improve conjugation efficiency
  • How to desalt your sample from reactants, reducing agents or stabilizers which could interfere with your reaction

To download your copy of the guide, fill out the form below for immediate access:

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