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Enzyme Guides

Enzyme Unit Definitions and Assay Design

Download a Free Copy This guide explains key concepts such as ‘enzyme units’ and ‘enzyme activity’ in simple terms and discusses enzyme assay design and the importance of operating in the ‘linear range’. There are also some tips on setting up assay controls and subtracting blanks. This guide covers: Definitions of enzyme units What is…

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Enzyme Conjugates for IVD Applications

Enzyme-antibody conjugates are key components of a variety of IVD products, including enzymelinked immunosorbent assay (ELISA) kits, lateral flow immunochromatographic devices, and kits based on immunohistochemistry and immunoblotting. The analyte of interest is detected by the antibody component of the conjugate, and measurability is provided by the enzyme. The assay signal is either proportional or…

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PiColorLock™ detection reagent

The phosphate detection reagent, PiColorLock™, changes color in the presence of inorganic phosphate (Pi) and this property can be exploited to measure the activity of any enzyme that generates Pi. The reagent is applicable to a large number of enzymes including ATPases, GTPases, phosphatases, heat-shock proteins and DNA unwinding proteins. The unique formulation of PiColorLock™…

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Maryel Gonzalez

Lightning-Link® Horseradish Peroxidase (HRP)

Lightning-Link® Horseradish Peroxidase (HRP)

We have used the kit to conjugates our molecules. the procedure is very simple. It gave much better results than other kits in the market.

Maryel Gonzalez

InstantBlue™ Protein Stain

InstantBlue™ Protein Stain

It is a wonderful product. It is very easy to use, no background. I will definitely recommend this product as staining for SDS-Page gels over…

Hannah Valentino

InstantBlue™ Protein Stain

InstantBlue™ Protein Stain

This product stains the protein gels very quickly with no background coloration. It is a fantastic time saver!

Will Michaels

RunBlue™ TEO-Tricine SDS Mini Gels

RunBlue™ TEO-Tricine SDS Mini Gels

Swapping to the TEO-Tricine gradient gels has allowed me to visualise the cleavage of a small peptide from a large MBP tag - whilst maintaining…

Capal

TruePrime® Single Cell WGA Kit

TruePrime® Single Cell WGA Kit

I've used this kit for amplification of DNA from single flow-sorted plant chromosomes. Performance was exactly as desribed, on average I've obtained more than microgram…

Ronald Magnusson

RunBlue™ Bis-Tris Protein Gels

RunBlue™ Bis-Tris Protein Gels

They run well - just need to be sure to get rid of bubbles under the gel.

Mari

TruePrime® Single Cell WGA Kit

TruePrime® Single Cell WGA Kit

I have used the kit for amplification of single cells extracted from bovine embryo. It worked well and the products were as expected. The products…

Boyan Grigorov

InstantBlue™ Protein Stain

InstantBlue™ Protein Stain

We have tried InstantBlue in order to have a rapid result and, yes, it works for 15 minutes as mentioned in the protocol. Before, students…

Michaela

Lightning-Link® R-Phycoerythrin (R-PE)

Lightning-Link® R-Phycoerythrin (R-PE)

We used this kit to label antibody for flow cytometry. It´s easy to use (our technician loves it) and the result is very good. As…