Protein Electrophoresis (Western Blotting) Technical Handbook
March 8th, 2019
Electrophoresis is a simple, rapid, and highly sensitive analytical technique to study the properties of proteins and nucleic acids, and has become a
The biological molecule of interest is contained in a sample that is generally run in a support matrix such as polyacrylamide gel or agarose. Polyacrylamide gel is widely employed to separate proteins, whereas agarose is mainly used to separate larger macromolecules such as nucleic acids. Polyacrylamide gel electrophoresis (PAGE) can be used to analyse the size, amount, purity, and isoelectric point of polypeptides and proteins.
One dimensional PAGE is a relatively simple and affordable technique. Sodium dodecyl
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