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pIMAGO-biotin

Quick Overview

- For Western Blot 
- HRP-based detection (ECL) 


pIMAGO is a universal phosphoprotein detection technology that enables sensitive and specific recognition of phosphorylated molecules. Unlike phospho-antibodies, the binding is not biased by amino acid sequence, and therefore can be used for detection of any phosphorylation event on any protein site. pIMAGO detection protocol resembles a simple Western Blot procedure and can be easily incorporated by any laboratory.

  • Catalog Number
  • Unit Size
  • MSDS
  • Product Manual
  • List Price
  • Qty
  • 900-100
  • HRP Colorimetric - Microplate - 100 wells
  • $154.00
  • 900-400
  • HRP Colorimetric - Microplate - 100 wells
  • $464.00
  • 901-100
  • Fluor 550 - Microplate - 100 wells
  • $154.00
  • 901-400
  • Fluor 550 - Microplate - 400 wells
  • $464.00
  • 902-100
  • Fluor 680 - Microplate - 100 wells
  • $154.00
  • 902-400
  • Fluor 680 - Microplate - 400 wells
  • $464.00
  • 903-100
  • Fluor 800 - Microplate - 100 wells
  • $154.00
  • 903-400
  • Fluor 800 - Microplate - 400 wells
  • $464.00
  • 800-10
  • HRP - Western Blot - 10 mini-blots
  • $149.00
  • 800-40
  • HRP - Western Blot - 40 mini-blots
  • $412.00
  • 801-10
  • Fluor 550 - Western Blot - 10 mini-blots
  • $149.00
  • 801-40
  • Fluor 550 - Western Blot - 40 mini-blots
  • $412.00
  • 802-10
  • Fluor 680 - Western Blot - 10 mini-blots
  • $149.00
  • 802-40
  • Fluor 680 - Western Blot - 40 mini-blots
  • $412.00
  • 803-10
  • Fluor 800 - Western Blot - 10 mini-blots
  • $149.00
  • 803-40
  • Fluor 800 - Western Blot - 40 mini-blots
  • $412.00

Results
pIMAGO analysis of 6 protein mixture (2 phosphoproteins) avidin-HRP or avidin-Fluor detection.

Avidin-HRP detection Avidin-Fluor680 detection
Amount of protein mix loaded (in ng) Amount of protein mix loaded (in ng)
Avidin-HRP detection Avidin-Fluor680 detection

pIMAGO analysis of in vitro kinase assays using avidin-HRP (left) or avidin-Fluor (right) detection.

JNK1-cJun kinase assay
JNK1-cJun kinase assay
pIMAGO
α-cJun
Overlay


Cdk1-Cdc6 kinase assay

Cdk1-Cdc6 kinase assay
Cdk1-Rb kinase assay
Cdk1-Rb kinase assay
pIMAGO
α-cJun
Overlay

 

Kit Components

Additional Information

Shelf Life 1 Year from receipt
Storage +4C