Unique Formulation for Enhanced Performance

PiColorLock™ phosphate detection reagent changes colour in the presence of inorganic phosphate (Pi). This can be exploited to measure any enzyme that generates Pi including ATPases, GTPases, phosphatases, heat shock proteins and DNA unwinding proteins.

The unique formulation of PiColorLock™ affords enhanced assay linearity, dynamic range and colour stability; it is also possible with PiColorLock™ to work with unstable substrates (e.g. ATP, GTP) that give high nonenzymatic background signals with other acidic dye based detection reagents.

Key benefits:

  • Colorimetric assay – Simple, scalable and nonradioactive
  • Compatible with almost any assay buffer – Flexibility over a wide range of existing assays
  • Stable reagent formulation – Long shelf life
  • Unique accelerator – Speeds up color development
  • Unique stabilizer – Suppresses nonenzymatic backgrounds with acid labile substrates (ATP or GTP)
  • PiColorLock™-Pi complex is very stable – No precipitation, results can be measured over several hours; ideal for HTS
  • Wide linear range – No inhibition of color development by high concentrations of protein
  • The unique PiColorLock™ Stabilizer prevents background drift with time.

PiColorLock™ phosphate detection system

The PiColorLock™ reagent is often used with unstable substrates (e.g. ATP, GTP) that give rise to nonenzymatic background drift with time. The unique stabilizer, provided in the PiColorLock™ phosphate detection system, blocks this nonenzymatic breakdown, so generating a stable low background. The stable signal can therefore be read up to several hours after the reaction has ended.

Feel free to contact us if you need more information regarding this product.

Demonstration of PiColorLock™ phosphate detection reagent performing homogeneously to outperform competitors

Figure 1. Competitor assays are beset with several problems including reagent precipitation. The high stability of PiColorLock™ ensures high stability of the colored dye–phosphate complexes (green color).

PiColorLock™ showing high stability of the colored dye-phosphate complexes compared to competitors

Figure 2. ATP has been incubated in three detection reagents. A steadily rising background signal is seen with competitor reagents, whereas PiColorLock™ provides baseline readings.

PiColorLock™ is designed to have a large linear range, reducing the need for sample dilution.

Figure 3. PiColorLock™ has also been designed to have a large linear range, thus reducing the need for sample dilution. Competitors’ products are linear over a much narrower range of concentrations.

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