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Immunoassays are the gold-standard method for the detection of proteins and biomarkers in discovery processes. However, ELISA development usually involves costly plate coating, antibody pair screening, long development and optimization times, and variable success rates. These limitations are now solved by CaptSure DIY ELISA.
CaptSure DIY ELISA is the latest cutting-edge ELISA kit with a built-in system for antibody labeling, enabling the rapid and easy development of in-house ELISAs using any antibody pair while eliminating the need to perform plate coating procedures.
Furthermore, the CaptSure DIY ELISA enables you to reduce the amount of capture antibody required, the ability to measure different targets on the same plate with different assay antibody pairs, and streamline your ELISA to a single-wash format if desired.
The CaptSure DIY ELISA optimized protocol results in a faster ELISA development, reduced overall costs, and a greater assay flexibility to increase the research output with a reduced chance of error.
How does CaptSure DIY ELISA work?
CaptSure DIY ELISA is powered by the combination of Expedeon’s proprietary CaptSure and Lightning-Link technologies.
Unlike standard ELISA systems, the CaptSure DIY ELISA kit has a ready-to-go CaptSure assay plate, pre-coated with our CaptSure antibody, which is specific for the CaptSure peptide. The capture antibody is quickly and easily conjugated with the Lightning-Link CaptSure peptide supplied within the kit. Once added to the plate, the interaction between the CaptSure antibody and the CaptSure peptide immobilizes the immuno-complex to the plate.
The detection antibody is similarly conjugated with HRP using the Lightning-Link HRP conjugation reagent, also supplied in the kit. Thus, with minimal hands-on time, you can set up both of your antibodies to ensure the best performance of your ELISA.
Lightning-Link is the world’s fastest conjugation technology to label antibodies and proteins by targeting free amine groups. As amine groups are ubiquitous and numerous in antibodies, any antibody can be labeled with the Lightning-Link CaptSure peptide and Lightning-Link HRP kits.
This labeling protocol is comprised of just a few simple steps:
- Resuspend the lyophilized Lightning-Link CaptSure peptide and Lightning-Link HRP with the solution of capture and detection antibodies, respectively;
- Incubate each at room temperature for the specified times;
- Quench the reactions, and your labeled capture and detection antibodies are ready to use for the ELISA.
CaptSure DIY ELISA workflow
The workflow of an ELISA utilizing the CaptSure system can be either multi-wash or single-wash, depending on your laboratory setup and desired level of optimization and automation.
In a multi-wash format, the assay sequence is very similar to that of a standard ELISA (bottom, left panel). The assay can, however, be simplified to a single-wash format, where the assay antibody concentrations are first optimized for the target concentration (bottom, right panel).
A more detailed diagram of a single-wash CaptSure ELISA is shown below. To set up your ELISA for a single-wash protocol, the concentration of CaptSure– and HRP- conjugated antibodies must first be optimized to be at the correct concentration for your analyte. Typically, this will be approximately 100-200 ng/mL final concentration in well of each antibody to a final volume of 100 μL in the well.
CaptSure DIY ELISA single-wash workflow
- Label your capture and detection antibodies of choice to Lightning-Link CaptSure peptide and Lightning-Link HRP, respectively
- Add the labeled antibody pair and the sample into the plate
- Incubate for one hour
- Wash the plate and detect
Work with us
Expedeon can provide assay development service and product validation, up to full product manufacture. For further information about collaboration, partnership, and licensing please get in contact with us.