Electrophoresis is dependent on running buffer, as it provides ions that carry a current. The electrical charge allows the separation of proteins, DNA and RNA for analysis, whilst also maintaining pH levels. This is significant as the charge of biomolecules can change as the pH fluctuates.
SDS PAGE relies on running buffer in the form of TEO-Tricine and Bis-Tris buffers, whilst blotting requires Western blot transfer buffer. All buffers have different solutions to enable optimum gel electrophoresis or protein transfer with their corresponding gel chemistry.
Our handy selection guide below lists each running buffer for each gel:
InstantBlot Transfer Buffer
We were previously doing our (wet) transfers for 90 minutes but have now cut this down to 30 minutes with the InstantBlot transfer buffer. Since…
RunBlue MOPS Run Buffer
I use MOPS buffer for my 4-12% bis-tris gels. The running process is fast and the bands are sharp. Since it came in a 20X…