RunBlue™ Tris-Glycine Blot & Transfer Buffer
RunBlue™ TGS Blot Buffer has been specially formulated to provide optimal blot transfer of proteins from RunBlue™ Precast Gels. Supplied as a concentrate, the solution should be diluted by a factor of ten for use with the RunBlue™ Dual Run and Blot system or a factor of 20 for use with other blotting methods.
• Transfer buffer for Tris-Glycine gel electrophoresis
RunBlue™ LDS Sample buffer (NXB31010) has been specifically formulated for use with RunBlue™ gels. The ions in the sample buffer match the gel buffer and the buffer has a higher density, making it compatible with the density of the running buffer. The buffer contains two tracking dyes. The red dye is designed to monitor the pH of the buffer. A colour change from red to yellow indicates a shift in pH indicating that the buffer was poorly made or recycled too many times.
RunBlue™ SDS Running Buffer (NXB50500) a nonreducing Tris-Tricine buffer system which provides a separation similar to the MOPS buffer used with Bis-Tris precast gels. It provides enhanced separation of higher molecular weight proteins. The buffer can also be used for reducing separation by adding antioxidant, such as RunBlue Antioxidant (NXA30010) to the buffer prior to use.
Simply choose the buffer that will provide the greatest resolution for your protein(s) of interest.
Take a look at our Sample Buffers and Membranes for Western blot.