RunBlue™ Tris-Glycine SDS Blot Buffer has been specially formulated to provide optimal blot transfer of proteins from RunBlue™ Precast Gels. Supplied as a concentrate, the solution should be diluted by a factor of ten for use with the RunBlue™ Dual Run and Blot system or a factor of 20 for use with other blotting methods.
RunBlue™ LDS Sample buffer (NXB31010) has been specifically formulated for use with RunBlue™ gels. The ions in the sample buffer match the gel buffer and the buffer has a higher density, making it compatible with the density of the running buffer. The buffer contains two tracking dyes. The red dye is designed to monitor the pH of the buffer. A color change from red to yellow indicates a shift in pH indicating that the buffer was poorly made or recycled too many times.
RunBlue™ SDS Running Buffer (NXB50500) a nonreducing Tris-Tricine buffer system that provides a separation similar to the MOPS buffer used with Bis-Tris precast gels. It provides enhanced separation of higher molecular weight proteins. The buffer can also be used for reducing separation by adding RunBlue™ Antioxidant (NXA30010) to the buffer prior to use.
All RunBlue™ Buffers, Reagents and Accessories have been specifically designed and formulated for use with the RunBlue™ gels and apparatus to provide optimum performance and results. Buffers are made using the highest quality chemicals and have different compositions to other manufacturers buffers.