Our DNA Ladders allow estimation of the molecular weight of linear double stranded DNA run in agarose gels. The 50bp PLUS ladder contains fragments from 50bp up to 1000bp, and the 50bp ladder contains fragments from 50bp to 500bp. Please see the product image for specific fragment lengths. The 50bp ladder contains 8 fragments, the 1kb PLUS contains 13 fragments.
Our DNA Ladders contain precisely quantified and measured linear double-stranded fragments of DNA pre-mixed with loading dye making them ready to use. Ladders have one or two fragments at a higher concentration to help identify specific bands in the ruler and estimate DNA length at a glance. Fragments are logical, round numbers with a sensible profile when run.
These ladders are formulated so that when loading 5 µl all fragments contain 40ng of DNA, apart from the higher concentration bands which will contain 100ng. Ladders are supplied in volumes of 500 µl, giving 100 runs worth of ladder if using 5µl per run. 2 – 5 µl recommended per run. Please see the following table for more specific information about individual ladders.
|Name (volume : 500 µl)||Band Range||Number of Fragments||Concentration (ng/µl)||Higher concentration bands|
|DNA Ladder - 1KB||0.5-10kb||10||104||2000, 5000|
|DNA Ladder PLUS - 1KB||0.1-10kb||15||144||500, 3000|
|DNA Ladder - 100bp||0.1-1.5kb||11||100||500|
|DNA Ladder PLUS - 100bp||0.1-3.0kb||14||136||500, 1000|
|DNA Ladder - 50bp||50-500bp||8||76||250|
|DNA Ladder PLUS - 50bp||50-1000bp||13||128||250, 500|
- Storage: +4C
- Shelf Life: 1 Year from receipt