RunBlue™ TriColor Prestained Protein Ladders
Our three color prestained protein marker has 13 prestained proteins ranging from 3.5 to 180 kDa in Molecular Weight (MW). Proteins are covalently coupled with a blue chromophore, except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS PAGE. The RunBlue™ Prestained Protein marker is designed for monitoring protein separation during SDS polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Recommended Load Volumes
RunBlue™ protein markers are provided ready to use in 1x RunBlue™ sample buffer. Recommended volumes to load are:
|Application||12 well volume||12 well loads / vial||17 well volume||17 well loads / vial|
|Monitor during Run||10 μl||60||5 μl||120|
|Visible after blotting||4 μl||150||2 μl||300|
|Visible after staining||4 μl||150||2 μl||300|
Stable for up to three months at 4°C.
For long term storage, store at -20°C.