RunBlue™ Prestained Protein Markers

RunBlue™ TriColor Prestained Protein Ladders

Our three color prestained protein marker has 13 prestained proteins ranging from 3.5 to 180 kDa in Molecular Weight (MW). Proteins are covalently coupled with a blue chromophore, except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS PAGE. The RunBlue™ Prestained Protein marker is designed for monitoring protein separation during SDS polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Recommended Load Volumes

RunBlue™ protein markers are provided ready to use in 1x RunBlue™ sample buffer. Recommended volumes to load are:

Application12 well volume
12 well loads / vial
17 well volume
17 well loads / vial
Monitor during Run
10 μl
60
5 μl
120
Visible after blotting
4 μl
150
2 μl
300
Visible after staining
4 μl
150
2 μl
300

Storage

Stable for up to three months at 4°C.
For long term storage, store at -20°C.

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