RunBlue™ Tri-colour Prestained Protein Ladders
Our three-color prestained protein marker has 12 pre-stained proteins ranging from 3.5 to 180 kDa in Molecular Weight (MW). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE. The RunBlue™ Prestained Protein marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
RunBlue™ Dual-color Prestained Protein Marker
The dual color protein ladder for SDS PAGE contains 10 recombinant proteins ranging in size from 7.6 kDa to 195 kDa. For easy visualization the 28 kDa and 71 kDa marker have been dyed orange, the other eight markers are blue. The purity and band sharpness mean the markers can be used for visualizing the separation while running the gel, checking transfer effectiveness of your western blot, or as a routine marker in place of other unstained markers.
Recommend Load Volumes
RunBlue™ protein markers are provided ready to use in 1x RunBlue™ sample buffer. Recommended volumes to load are:
|Application||12 well volume||12 well loads/vial||17 well volume||17 well loads/vial|
|Monitor during Run||10 μl||60||5 μl||120|
|Visible after blotting||4 μl||150||2 μl||300|
|Visible after staining||4 μl||150||2 μl||300|
Stable for up to 3 months at 4°C.
For long-term storage, store at -20°C.