RunBlue™ Bis-Tris Protein Gels
The Protein Gels (10x10cm) have been developed by Expedeon to provide a more cost effective alternative to NuPAGE® Bis-Tris gels. The RunBlue™ Bis-Tris gels run with the same voltages and times, in the same tanks, using the same MES / MOPS buffers as NuPAGE® Bis-Tris gels and achieve highly comparable results with exceptional reproducibility. RunBlue™ Bis-Tris Protein Gels also benefit from the usability enhancements present in all RunBlue™ gels such as comb / strip free design and tear proof composition.
Key Benefits of RunBlue™ Bis-Tris:
- Standard Bis-Tris buffers – Compatible with MES and MOPS buffers
- Accepted standard format – Compatible with tanks such as NuPAGE® XCell SureLock™ Mini-Cell
- Standard Bis-Tris protocol – Fast run times at 200V
- Neutral running pH – Sharp bands and high protein integrity
- Unique homogeneous polymerization – Increased consistency and no residual free acrylamide
- Extra wells – Run up to 12 or 17 samples simultaneously
- Deep and wide wells – Load up to 35 μl (12 well) or 20 μl (17 well)
- Protruding teeth – No well contamination in case of overloading
- Resilient gel matrix – Tear proof gel and risk free handling
- Comb free – Reduced risk of broken or damaged teeth
- Strip free – No potentially ruined Western blots.
- Neutral buffer and high chemical stability – Shelf life of 1 year at 4°C and 3 months at ambient temperature.
RunBlue™ Bis-Tris Protein Gels are based on Bis-Tris neutral gel buffer system, providing a migration profile highly comparable to NuPAGE® Bis-Tris gels. The Bis-Tris Protein Gels have an approximate range of 3.5kDa to 160kDa with MES Buffer and 30kDa to 180kDa when using MOPS. RunBlue™ Protein Gel’s proprietary polymerization process results in more uniform gels between batches, with decreased variability and improved repeatability of results.
In addition to their high resolution separation of protein, all RunBlue™ Protein Gels have a number of enhancements to improve and streamline usability. RunBlue™ Protein Gels are up to 10 times stronger than conventional hand cast gels and can stand up to repeated handling. This virtually eliminates the risk of torn and unusable gels encountered during normal gel manipulation. More lanes than standard gels allows more proteins to be run and wide, deep wells allow higher volumes to be loaded.
Cassettes have no comb or tape to remove and no excess gel to trim, all of which improves the speed and efficiency of the loading and running process. Wells are outlined and numbered for quicker sample loading and the cassette is labeled with gel type, percentage and a unique lot number for traceability. Well teeth protrude above the cassette to prevent sample contamination between wells in case of overloading.
These gels are compatible with Invitrogen® XCell SureLock™ Mini-Cell equipment. For using these gels with any other kind of tank, please contact us first.
They also have a three month shelf life at ambient temperature, which is convenient when fridge space is limited, and one year at 4°C, which is useful when gel requirements are low or variable.
RunBlue™ Bis-Tris Protein Gels must be run with MES or MOPS buffers such as RunBlue™ MES Running Buffer and RunBlue™ MOPS Running Buffer, which are available as a premixed 20X solution, and the samples must be prepared with a Tris-HCl LDS sample buffer such as RunBlue™ LDS Bis-Tris Sample buffer, which is available as a premade 4X solution.
Usage of MES or MOPS will vary depending on the molecular weight range of proteins to be resolved. RunBlue™ Bis-Tris Protein Gels are also compatible with NuPAGE® MES and MOPS buffers and NuPAGE® LDS Sample Buffer.
Polyacrylamide gels are based on the polymerization of liquid acrylamide and bis-acrylamide. The polymerization is usually induced by the addition of a source of free radicals and a stabilizer (usually APS & TEMED), which induces cross link formation between acrylamide and bis-acrylamide monomers. This localized chemical induction results in heterogeneous polymerization across the gel due to the acrylamide concentration gradients being generated, and can lead to the presence of residual free acrylamide at the end of the polymerization.
Expedeon has developed a unique physical induction technology that ensures homogeneous polymerization across the whole gel and no residual, acrylamide free after the end of the polymerization. Numerous studies have shown the acrylamide monomer to be a neurotoxin and possible carcinogen*. Acrylamide can be inhaled as a dry powder, evaporate from liquid solution and sublimate from solid gels if not fully polymerized. RunBlue gels are polymerized in a unique homogeneous manner that results in a consistent gel with minimal or no residue after casting.
* AIHA J (Fairfax, Va). 2002 Jul-Aug;63(4):468-73. Inhalation exposures to acrylamide in biomedical laboratories.