RunBlue™ TEO-Tricine SDS Mini Gels

The RunBlue™ TEO-Tricine SDS Mini Gels (10x8cm cassettes) are designed to fit a number of gel tanks such as Bio-Rad Mini-PROTEAN Tetra Cell. RunBlue™ TEO-Tricine SDS Protein Gels are highly optimized for a TEO-Tricine buffer system and must be run with this buffer system to achieve good results.

Expedeons’ TEO-Tricine gels should be run with our TEO-Cl formulated buffers; RunBlue™ SDS Running Buffer and RunBlue™ LDS Sample Buffer.

All RunBlue™ Protein Gels have an extended shelf life of two years at 4ºC, which is ideal for large stocks or infrequent use. They also have a 6-month shelf life at ambient temperature, which is convenient when fridge space is limited.

Key RunBlue™ Benefits

  • Novel optimised gel–buffer system – High resolution and sharp bands
  • Extra resolving length – Increased separation
  • Unique homogeneous polymerization – Increased consistency and no residual free acrylamide
  • Extra wells – Run up to 12 or 17 samples simultaneously
  • Deep and wide wells – Load up to 35 μl (12 well) or 20 μl (17 well)
  • Standard format – Compatible with 10x8cm protein gel tanks such as Bio-Rad Mini-PROTEAN models
  • Protruding teeth – No well contamination in case of overloading
  • Resilient gel matrix – Tear proof gel and risk free handling
  • Comb free – No potentially broken or damaged teeth
  • Strip free – No potentially ruined Western blots
  • Clipped cassette – Fast and gentle opening
  • Neutral buffer and high chemical stability – Shelf life of 2 years at 4ºC and 6 months at ambient temperature.

RunBlue™ Performance

RunBlue™ TEO-Tricine SDS Mini Gels take advantage of Expedeon’s new methods and innovations to the gel making process to increase resolution and reproducibility of separated protein bands. RunBlue™ TEO-Tricine SDS Mini Gels are based on a novel gel buffer system, which provides a separation profile similar to Mini-PROTEAN® TGX™ Precast Gels, but with enhanced separation of higher molecular weight proteins and better overall resolution. RunBlue™ Protein Gel’s proprietary polymerization process results in more uniform gels between batches, with decreased variability and improved repeatability of results. Our RunBlue™ TEO-Tricine SDS Mini Gels are also available in 10x10cm sizes.

Usability Enhancements

In addition to their high resolution separation of protein all RunBlue™ Protein Gels have a number of enhancements to improve and streamline usability. RunBlue™ Protein Gels are up to 10 times stronger than conventional hand cast gels and can stand up to repeated handling. This virtually eliminates the risk of torn and unusable gels encountered during normal gel manipulation. More lanes than standard gels allows more proteins to be run and wide, deep wells allow higher volumes to be loaded.

Cassettes have no comb or tape to remove and no excess gel to trim, all of which improves the speed and efficiency of the loading and running process. Wells are outlined and numbered for quicker sample loading and the cassette is labeled with gel type, percentage and a unique lot number for traceability. Well teeth protrude above the cassette to prevent sample contamination between wells in case of overloading.

Improved Safety

Polyacrylamide gels are based on the polymerization of liquid acrylamide and bis-acrylamide. The polymerization is usually induced by the addition of a source of free radicals and a stabilizer (usually APS & TEMED), which induces cross link formation between acrylamide and bis-acrylamide monomers. This localized chemical induction results in heterogeneous polymerization across the gel due to acrylamide concentration gradients being generated, and can lead to the presence of residual free acrylamide at the end of the polymerization.

Expedeon has developed a unique physical induction technology which ensure homogeneous polymerization across the whole gel and no residual, free acrylamide after the end of the polymerization. Numerous studies have shown the acrylamide monomer to be a neurotoxin and possible carcinogen*. Acrylamide can be inhaled as a dry powder, evaporate from liquid solution and sublimate from solid gels if not fully polymerized. RunBlue gels are polymerized in a unique homogeneous manner that results in a consistent gel with minimal or no residue after casting.

* AIHA J (Fairfax, Va). 2002 Jul-Aug;63(4):468-73. Inhalation exposures to acrylamide in biomedical laboratories.

Gel Migration Chart

Teo-Tricine SDS Mini Gel Migration Chart displaying proteins from 6.5kDa to 200kDa

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