CovCheck™ Genome Coverage WGA QC Multiplex Kit

PCR Kit for Determining Human Single Cell Whole Genome Amplification Success

CovCheck™ Genome Coverage WGA QC Multiplex Kit provides a ready to use set of endpoint PCR primers in a convenient 96 well plate format, complete with optimized PCR reagents including a premium hot start Taq polymerase. The plate includes 16 identical sets of 24 different primer pairs multiplexed in six reactions, that will amplify small portions from each human chromosome, allowing the coverage analysis of 16 independent single cell whole genome amplifications simultaneously.

Features & Benefits:

  • High throughput check of WGA sample amplification coverage
  • Only sequence successful amplifications
  • Ready to use 96 well PCR panel
  • Avoid long waits and wasted money.

Additional Information:

  • Storage: -20°C
  • Shelf life: Six Months from receipt.

Grant Information

CovCheck Multiplex kit - PCR gel results

Figure 1. CovCheck™ Multiplex results obtained with human genomic DNA (male and female), and DNA amplified with TruePrime™ from single HEK293 cells. Each group (1–6) shows four different amplicons located in four different chromosomes.

CovCheck Multiplex Kit - Percentage of positive hits graph

Figure 2. Correlation between CovCheck™ SinglePlex and Multiplex kits. The correlation between the results obtained with each of the 16 single HEK293 cells used in the kits is statistically significant (p<0.01)

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