qPCR CovCheck™ Genome Coverage WGA QC SinglePlex Kit

Whole Genome Amplification Real Time Coverage Analysis

A ready to use set of PCR primers in a convenient 96 well plate format, paired with optimized qPCR reagents. The plate includes four identical sets of 24 different primer pairs that will amplify small portions from each human chromosome, whilst allowing the real time coverage analysis of four independent single cell whole genome amplifications.

The optimized qPCR reagents (e.g. qCovCheckTM Master Mix: Hot Start Taq DNA polymerase, SYBR Green I, reaction buffer and dNTPs) mean that the qPCR CovCheckTM kit is an easy and convenient approach to getting the best from your single cell experiments, by enabling visualization of specific targets whilst minimizing costs.

Human genome coverage obtained by sequencing versus qPCR CovCheck™ results.

 

Graph depicting the correlation between Poisson distribution deviations and CovCheck qPCR panel coverage.

Human genome coverage obtained by sequencing versus qPCR CovCheck™ results.

Excellent correlation between qPCR CovCheck™ kit results and results generated through sequencing.

Agarose gel electrophoresis of the qPCR CovCheck™ amplicons

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