Endonuclease for Nucleic Acid Degradation and Removal
Endonuclease enzyme BaseMuncher is a nonspecific endonuclease that is used for the removal of nucleic acid from protein samples. Our ultrapure endonuclease hydrolyzes both single stranded and double stranded, along with circular and linear nucleic acids (DNA and RNA) to 5-phosphorylated oligonucleotides of 1–4 bases in length.
Recombinantly produced in E. coli using the Benzonase gene in a proprietary process, BaseMuncher is a highly purified homodimer of 27kDa subunits that have exceptionally high specific activity and is completely free of protease activity. BaseMuncher is ideal for reducing viscosity during protein purification and sample preparation.
One Unit of BaseMuncher converts 1.0 OD260 of salmon sperm DNA into acid soluble nucleotides in 30 minutes at 37°C in a reaction buffer of 50mM Tris-HCl, pH 8.0 and 1mM MgCl2. This corresponds to complete digestion of 50g of salmon sperm DNA into oligonucleotides.
Features & Benefits:
- Exceptionally high endonuclease specificity for DNA and RNA
- Ultrapure to >99%
- Free of protease activity
- High purity and high concentration grade
- Easy protein purification –Reduced viscosity during sample preparation
- 100x greater activity than DNAase
- Cheaper than Benzonase; but the same quality and efficiency.
- Storage: +4°C
- Shelf Life: One year from receipt.