SunScript™ One Step RT-PCR Kit is an easy and reliable system designed for fast RT-PCR reactions using a novel Reverse Transcriptase.
A key advantage of SunScript is the elevated temperature for performing the RT step thus allowing higher specificity of the reaction, and a higher likelihood in obtaining reaction products for difficult targets. Moreover, the excellent kinetic performance of the SunScript™ Reverse Transcriptase allows for speed optimization of the protocol. The kit contains all the components needed to perform reverse transcription PCR amplification. The Reverse Transcriptase step can be completed in as little as 5 minutes.
SunScript™ One Step RT-PCR Kit is supplied in a single box, with sufficient amount for either 25 or 100 reactions. Contents are provided in clear, screw-cap tubes with colour-coded lids as referenced under ‘Contents’ at the end of this document.
Shipping and Storage
This product is shipped on dry ice. Upon receipt, it should be stored immediately at -20ºC in a non-frost-free (constant temperature) freezer. If stored correctly, the product can be kept for at least three months after shipping without displaying any reduction in performance. For longer periods of time, store the kit at -80ºC. Avoid frequent freeze-thaw cycles. Avoid longer exposure of kit contents to light.
The system uses an enzyme master mix containing SunScript™ Reverse Transcriptase RNaseH-, a high quality HotStart Taq Polymerase, RNase inhibitor, and a 2X buffer containing dNTPs, salts, stabilizers and the double stranded DNA-binding dye.
SunScript™ Reverse Transcriptase RNaseH- is an engineered version of the well characterized HIV-1 RT with increased thermostability. This enables to perform the reverse transcription step at temperatures up to 75ºC if needed (the recommended temperature for the RT reaction is 60ºC for most samples depending on the melting temperature of the primers). These features make this kit the best choice for amplifying a broad range of mRNAs, also with complicated secondary structures or high GC content. The reverse transcription reaction can be effectively performed while Taq Polymerase remains inactive during this step due to blocking antibodies. The inactivation/activation step at 95ºC provides an automatic ‘hot start’ for the Taq Polymerase to amplify the reverse transcribed cDNA.
The reaction mixes contain proprietary formulations which have been optimized for both the reverse transcription and the PCR reaction, including dNTPs, MgCl2 and stabilizers.
Each batch of SunScript™ One Step RT-PCR Kit and individual components are tested against predetermined specifications to ensure consistent product quality.
SunScript™ One Step RT-PCR Kit is intended for molecular biology use only and in vitro use only. This product is not intended for diagnosis, prevention or treatment of a disease in human beings or animals.
SunScript™ is a trademark of SYGNIS AG or one of its subsidiaries (SYGNIS BIOSCIENCE GmbH & Co. KG or SYGNIS BIOTECH, S.L.U.). All rights reserved.
SunScript™ One Step RT-PCR Kit includes SunScript™ Reverse Transcriptase RNaseH+. Patent protection is pending for SunScript™ Reverse Transcriptase RNaseH- and methods associated with its use.
SunScript™ One Step RT-PCR Kit includes a non-transferable license to use SunScript™ Reverse Transcriptase RNaseH- and the SunScript™ RT-PCR kit for the purchaser’s internal research. All other commercial uses of this product, including without limitation product use for diagnostic purposes, resale of product in the original or any modified form or product use in providing commercial services require a separate license.
The purchase of this product includes a limited, non-transferable license for all fields other than human or veterinary in vitro diagnostics under specific claims of U.S. Patent Nos. 6,174,670 and 6,569,627, owned by the University of Utah Research Foundation and licensed to BioFire Defense, LLC and Roche Diagnostics GmbH, to use only the enclosed amount of product according to the specified protocols. No right is conveyed, expressly, by implication, or by estoppel, to use any instrument or system under any claim of U.S. Patent Nos. 6,174,670 and 6,569,627, other than for the amount of product contained herein.
Safety data sheets are available for download. This material may contain substances or activities that are harmful to human health. It should not be ingested, inhaled, or brought into contact with skin, and handled with appropriate care in accordance with the principles of good laboratory practices.
|Cap color||100 reactions|
|Enzyme MIX||Purple||1 x 45 μl|
|2x Reaction Buffer||Yellow||1 x 1000 μl|
|H2O||Blue||1 x 1000 μl|