SunScript Reverse Transcriptase

SunScript™ Reverse Transcriptase RNaseH- is a reverse transcriptase that has been engineered for high thermostability and absence of RNaseH activity based on the well characterized HIV-1 RT. It has RNA and DNA-dependent polymerase activity. The enzyme is active up to temperatures of 85°C. The elimination of RNaseH activity provides this enzyme with the best performance to allow high yields of full length cDNA synthesis on long RNA molecules with complex secondary structures. SunScript can completely synthesise cDNA of up to 11 Kb in length in only 5 minutes.

Supply
SunScript™ Reverse Transcriptase RNaseH- is supplied in a box, with sufficient amount for either 10, 50 or 200 reactions. The box also includes 5X Reaction Buffer and 0.1 M DTT. Contents are provided in clear, screw-cap tubes with colour-coded lids as referenced at Contents at the end of this document.

Shipping and Storage
This product is shipped in dry ice. Upon receipt, it should be stored immediately at -20°C in a non-frost-free (constant temperature) freezer. If stored correctly, the product can be kept for at least six months after shipping without displaying any reduction in performance. For longer periods of time, store the kit at -80°C.

Technical specifications
SunScript™ Reverse Transcriptase RNaseH- has been tested to be free of endonucleases, exonucleases and ribonucleases. The purity of the enzyme is guaranteed to be higher than 95%. The enzyme and reagents have been tested for amplification of 16 Kb first strand cDNA for 60 min at 65°C.

Quality Control
Each batch of SunScript™ Reverse Transcriptase RNaseH- is tested against predetermined specifications to ensure consistent product quality.

Intended use
SunScript™ Reverse Transcriptase RNaseH- is intended for molecular biology use only and in vitro use only. This product is not intended for diagnosis, prevention or treatment of a disease in human beings or animals.

Patent
Patent protection pending for SunScript™ Reverse Transcriptase RNaseH- and methods associated with its use.

Safety information:
Safety data sheets are available for download from www.sygnis.com. This material may contain substances or activities that are harmful to human health. It should not be ingested, inhaled, or brought into contact with skin, and handled with appropriate care in accordance with the principles of good laboratory practices. DTT is irritating (to eyes, respiratory system and skin), and harmful if swallowed. Risk and safety phrases: (R22, R36/37/38).

Contents

Description Cap color 10 reactions 50 reactions 200 reactions
SunScript™ RT RNaseH- Orange 1 x 15 µl 1 x 75 µl 1 x 300 µl
5X Reaction Buffer Yellow 1 x 40 µl 1 x 200 µl 1 x 800 µl
DTT White 1 x 20 µl 1 x 100 µl 1 x 400 µl
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