SunScript™ Reverse Transcriptase is a reverse transcriptase that has been engineered for high thermostability and absence of RNaseH activity . It has RNA and DNA-dependent polymerase activity. The enzyme is active up to temperatures of 85°C. The elimination of RNaseH activity provides this enzyme with the best performance to allow high yields of full length cDNA synthesis on long RNA molecules with complex secondary structures. SunScript™ can completely synthesise cDNA of up to 11 Kb in length in only 5 minutes.
SunScript™ Reverse Transcriptase is supplied in a box, with sufficient amount for either 10, 50 or 200 reactions. The box also includes 5X Reaction Buffer and 0.1 M DTT. Contents are provided in clear, screw-cap tubes with colour-coded lids as referenced in the table below.
Shipping and Storage
This product is shipped in dry ice. Upon receipt, it should be stored immediately at -20°C in a non-frost-free (constant temperature) freezer. If stored correctly, the product can be kept for at least six months after shipping without displaying any reduction in performance. For longer periods of time, store the kit at -80°C.
SunScript™ Reverse Transcriptase has been tested to be free of endonucleases, exonucleases and ribonucleases. The purity of the enzyme is guaranteed to be higher than 95%. The enzyme and reagents have been tested for amplification of 16 Kb first strand cDNA for 60 min at 65°C.
Each batch of SunScript™ Reverse Transcriptase is tested against predetermined specifications to ensure consistent product quality.
SunScript™ Reverse Transcriptase is intended for molecular biology and in vitro use only.
|Description||Cap color||10 reactions||50 reactions||200 reactions|
|SunScript™ RT RNaseH-||Orange||1 x 15 µl||1 x 75 µl||1 x 300 µl|
|5X Reaction Buffer||Yellow||1 x 40 µl||1 x 200 µl||1 x 800 µl|
|DTT||White||1 x 20 µl||1 x 100 µl||1 x 400 µl|