TruePrimeTM Necrotic cell-free/exosomal DNA amplification kit
For the amplification of genomic DNA starting from cell-free DNA obtained from plasma, serum, urine or any other bodily fluid.
The TruePrime™ Necrotic cell-free/exosomal DNA amplification kit uses a novel multiple displacement amplification method based on the combination of the recently discovered DNA primase TthPrimPol, and the highly processive and high-fidelity Phi29 DNA polymerase, to amplify genomic DNA starting from cell-free DNA* obtained from plasma, serum, urine or any other bodily fluid. The strong strand displacement capacity of Phi29 DNA polymerase allows TthPrimPol to generate new primers on the displaced strands that are extended by Phi29 DNA pol, resulting in exponential isothermal DNA amplification.
Features and benefits
• Excellent sensitivity and reproducibility
• Does not generate primer artefacts
• Excellent coverage with little bias
• Preserves SNVs and SNV frequencies across different sequencing technologies
TruePrime™-mediated amplification will preferentially amplify and therefore select the larger, more cancer-specific, DNA fragment size population from the cell-free DNA prep. Cell-free DNA molecules larger than 1 kb can be derived from necrotic cell death or extracellular vesicles like exosomes. This kit provides a method for assessing cancer genome information from all samples, regardless of tumour, with superior sensitivity and specificity. TruePrime Necrotic cell-free/exosomal DNA amplification kit is reliable at the level of 100 pg of cfDNA input, preserving the SNVs and SNVs frequencies across different sequencing technologies and allowing the amplification of cfDNA to levels that permit reliable, high depth sequencing.
TruePrime Necrotic cell-free/exosomal DNA amplification kit addresses the issue of liquid biopsy sensitivity and specificity by an adapted amplification of cancer-disease specific large fragment cell-free DNA.
*please note this kit cannot be used for the amplification of apoptotic DNA (160-170 bp), please see our TruePrime™ apoptotic cell-free DNA amplification kit.
Shipping and storage
The TruePrimeTM Necrotic cell-free/exosomal DNA amplification kit is shipped in dry ice. Upon receipt, the kit should be stored immediately at -20°C in a non-frost-free (constant temperature) freezer. If stored correctly the product can be kept for at least 24 months after shipping without displaying any reduction in performance. For longer storage periods we recommend a temperature of -80oC.