TruePrime® Necrotic-Cell Free / Exosomal DNA Amplification Kit
The TruePrime® Necrotic Cell-Free / Exosomal DNA Amplification kit preferentially amplifies larger (≥1kb) DNA fragments derived through necrosis or from extracellular vesicles like exosomes. This presents a method for assessing cancer genome information regardless of tumour, with superior sensitivity (100 pg cfDNA input) and specificity.
Note: This kit cannot be used for the amplification of apoptotic cell-free DNA (160-170 bp). For this we would recommend the TruePrime® apoptotic cell-free DNA amplification kit.
|Specific amplification of large DNA fragments (≥1kb)||Optimized kit and protocol for large templates (time saving)|
|Excellent sensitivity and reproducibility||Perfect for precious / rare samples|
|Primer-free amplification – TthPrimPol synthesizes the primers for Phi29 DNA polymerase||No primer-associated artefacts such as primer dimers – better representivity (cost and time saving)|
|Excellent coverage with minimal bias||Reliable results for publication|
|Wide variety of cell-free DNA sample sources:|
• Other bodily fluids
|Less restriction in sample source – greater experimental flexibility|
|Reliable at a cfDNA input level of 100 pg, preserving SNVs and SNV frequencies across different sequencing technologies||Amplification product sequencing compatibility|
- Assessing cancer genome information from all samples, regardless of tumour, with superior sensitivity and specificity
- Genetic research using large (≥1kb) circulating cell-free DNA
- Cancer research
- Amplification of large (≥1kb) DNA